Bonet Sergi, Delgado-Bermúdez Ariadna, Yeste Marc, Pinart Elisabeth
Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17003, Girona, Spain.
Biotechnology of Animal and Human Reproduction (TechnoSperm), Department of Biology, Institute of Food and Agricultural Technology, University of Girona, E-17003, Girona, Spain.
Anim Reprod Sci. 2018 Oct;197:134-144. doi: 10.1016/j.anireprosci.2018.08.021. Epub 2018 Aug 18.
The present study analyses the interaction of boar spermatozoa with Escherichia coli and Clostridium perfringens, in 9-day refrigerated semen samples. Ejaculates from 10 sexually mature boars were inoculated with either E. coli or C. perfringens, at infective titer from 10 to 10 CFU/mL. The sperm-bacteria interaction was evaluated by optical and scanning electron microscopy (SEM), through: a) determining the proportion of spermatozoa with either E. coli or C. perfringens adhered and the number and localization of bacteria adhered on the sperm surface; and b) analyzing the effects of bacterial contamination on sperm membrane integrity. While proportions of spermatozoa with bacteria adhered did not differ between E. coli and C. perfringens during the first 3 days of storage, proportions of spermatozoa with E. coli adhered were greater than those with C. perfringens on and subsequent to Day 4 of storage. Both bacterial types adhered non-specifically over the sperm surface; nevertheless, the proportion of spermatozoa with bacteria adhered to the mid-piece was greater with E. coli infection, and of spermatozoa with bacteria adhered to the principal piece greater with C. perfringens infections. Alterations in the sperm plasma membrane induced by C. perfringens affected the acrosomal end and appeared at Day 4 of storage, those induced by E. coli were apparent from Day 7 of storage and affected the acrosomal region and mid-piece. In conclusion, E. coli has a greater capacity to adhere to the sperm surface than C. perfringens during liquid storage at 17 °C, but damage on sperm membrane integrity induced by C. perfringens occurred in a shorter period of time.
本研究分析了在9天冷藏精液样本中，公猪精子与大肠杆菌和产气荚膜梭菌的相互作用。将来自10头性成熟公猪的射精样本接种大肠杆菌或产气荚膜梭菌，感染滴度为10至10 CFU/mL。通过光学显微镜和扫描电子显微镜（SEM）评估精子与细菌的相互作用，具体如下：a）确定附着有大肠杆菌或产气荚膜梭菌的精子比例，以及附着在精子表面的细菌数量和定位；b）分析细菌污染对精子膜完整性的影响。在储存的前3天，附着有细菌的精子比例在大肠杆菌和产气荚膜梭菌之间没有差异，但在储存第4天及之后，附着有大肠杆菌的精子比例高于附着有产气荚膜梭菌的精子比例。两种细菌均非特异性地附着在精子表面；然而，大肠杆菌感染时，附着在中段的带菌精子比例更高，产气荚膜梭菌感染时，附着在主段的带菌精子比例更高。产气荚膜梭菌诱导的精子质膜改变影响顶体末端，在储存第4天出现，大肠杆菌诱导的改变从储存第7天开始明显，影响顶体区域和中段。总之，在17°C液体储存期间，大肠杆菌比产气荚膜梭菌具有更强的附着于精子表面的能力，但产气荚膜梭菌诱导的精子膜完整性损伤发生时间更短。
