Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology, Faculty of Sciences, University of Girona, C/Maria Aurèlia Campany, 69, Campus de Montilivi, 17003, Girona, Spain.
Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology, Faculty of Sciences, University of Girona, C/Maria Aurèlia Campany, 69, Campus de Montilivi, 17003, Girona, Spain.
Anim Reprod Sci. 2020 May;216:106349. doi: 10.1016/j.anireprosci.2020.106349. Epub 2020 Apr 9.
This study evaluated how Proteus vulgaris affects sperm quality and sperm-bacteria interaction in stored semen samples. A strain of P. vulgaris resistant to streptomycin, penicillin, lincomycin and spectinomycin was added to boar semen in doses of 10, 10, 10, 10 and 10 CFU/mL. A sample in which there was no addition of P. vulgaris was the negative control. Sperm quality was determined by evaluating sperm motility and morphology using the computer-assisted sperm analysis (CASA) system, and plasma membrane and acrosome integrity using flow cytometry at 0, 1, 2, 4, 6, 8 and 10 days of liquid-storage at 17 °C. At the same time points, pH, sperm agglutination, sperm-bacteria interaction and bacterial growth were also assessed. There was impaired sperm motility when the infective doses of P. vulgaris were equal to or greater than 10 CFU/mL with the effect being dose-dependent (P < 0.05). When infective doses of P. vulgaris were of 10 to 10 CFU/mL there were impairments of plasma membrane and acrosome integrity by Day 1 of storage (P < 0.05), and alkalinisation of the storing medium by Day 4 (P < 0.05). Bacterial adhesion increased when infective dose of P. vulgaris was greater and as duration of storage increased. P. vulgaris had a high affinity for the mid- and principal pieces of sperm cells. It is concluded there were alterations in sperm motility in samples infected with P. vulgaris that were associated with bacterial adhesion and medium alkalinisation.
本研究评估了普通变形菌对贮存精液样品中精子质量和精子-细菌相互作用的影响。将 10、10、10、10 和 10 CFU/mL 剂量的耐链霉素、青霉素、林可霉素和大观霉素的普通变形菌菌株添加到猪精液中。未添加普通变形菌的样品作为阴性对照。使用计算机辅助精子分析 (CASA) 系统评估精子活力和形态，通过流式细胞术评估精子质膜和顶体完整性，在 17°C 下贮存 0、1、2、4、6、8 和 10 天，对精子质量进行了评估。在相同的时间点，还评估了 pH 值、精子凝集、精子-细菌相互作用和细菌生长。当普通变形菌的感染剂量等于或大于 10 CFU/mL 时，精子活力受损，且呈剂量依赖性（P < 0.05）。当感染剂量为 10 至 10 CFU/mL 时，在贮存第 1 天质膜和顶体完整性受损（P < 0.05），在贮存第 4 天贮存介质碱化（P < 0.05）。当普通变形菌的感染剂量增加和贮存时间延长时，细菌黏附增加。普通变形菌对精子的中部和主部具有很高的亲和力。研究结论是，感染普通变形菌的样本中精子活力发生改变，与细菌黏附和介质碱化有关。
