Toloczyki C, Feix G
Nucleic Acids Res. 1986 Jun 25;14(12):4969-86. doi: 10.1093/nar/14.12.4969.
A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two prominent S1 mapping signals. The sequence of a typical repeat unit is compared to a corresponding 130 bp long wheat repeat unit. The possible functional relevance of the repeat units is discussed.
分离出一个携带核rRNA基因和间隔区的14kb玉米DNA片段,并通过限制性酶切图谱对其进行了表征。对一个完整的3020bp长的外部间隔区进行了测序,发现有9个串联排列的200bp长的重复单元,具有高度同源性。这些重复单元位于两个突出的S1图谱信号的上游。将一个典型重复单元的序列与一个相应的130bp长的小麦重复单元进行了比较。讨论了这些重复单元可能的功能相关性。