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物种特异性核糖体DNA转录是由启动子特异性结合因子引起的。

Species-specific rDNA transcription is due to promoter-specific binding factors.

作者信息

Miesfeld R, Arnheim N

出版信息

Mol Cell Biol. 1984 Feb;4(2):221-7. doi: 10.1128/mcb.4.2.221-227.1984.

DOI:10.1128/mcb.4.2.221-227.1984
PMID:6700588
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368684/
Abstract

RNA polymerase I transcription factors were purified from HeLa and mouse L cell extracts by phosphocellulose chromatography. Three fractions from each species were found to be required for transcription. One of these fractions, virtually devoid of RNA polymerase I activity, was found to form a stable preinitiation complex with small DNA fragments containing promoter sequences from the homologous but not the heterologous species. These species-specific DNA-binding factors can explain nucleolar dominance in vivo in mouse-human hybrid somatic cells and species specificity in cell-free, RNA polymerase I-dependent transcription systems. The evolution of species-specific transcriptional control signals may be the natural outcome of a special relationship that exists between the RNA polymerase I transcription machinery and the multigene family coding for rRNA.

摘要

通过磷酸纤维素色谱法从人宫颈癌（HeLa）细胞和小鼠L细胞提取物中纯化RNA聚合酶I转录因子。发现每个物种的三个组分是转录所必需的。其中一个组分几乎没有RNA聚合酶I活性，发现它能与含有来自同源而非异源物种启动子序列的小DNA片段形成稳定的预起始复合物。这些物种特异性的DNA结合因子可以解释小鼠 - 人杂交体细胞体内的核仁显性以及无细胞的、RNA聚合酶I依赖性转录系统中的物种特异性。物种特异性转录控制信号的进化可能是RNA聚合酶I转录机制与编码rRNA的多基因家族之间存在的特殊关系的自然结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/292c104b1724/molcellb00144-0013-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/ec105ed488d1/molcellb00144-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/632dba2332b4/molcellb00144-0011-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/d5aa02efb31b/molcellb00144-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/0936ed878237/molcellb00144-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/292c104b1724/molcellb00144-0013-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/ec105ed488d1/molcellb00144-0010-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/632dba2332b4/molcellb00144-0011-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/d5aa02efb31b/molcellb00144-0012-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/0936ed878237/molcellb00144-0013-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18f5/368684/292c104b1724/molcellb00144-0013-b.jpg

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