Griess E A, Grasser K D, Feix G
Institut für Biologie III, Albert-Ludwigs-Universität Freiburg, FRG.
Planta. 1993;191(4):524-31. doi: 10.1007/BF00195754.
The 3-kb external spacer from a maize (Zea mays L. cv. A619) nuclear rRNA gene unit which contains nine highly homologous 200-bp repeat elements was found to include a region with DNA-curvature properties. The centre of curvature was localized within repeats 5 and 6 using a circular permutation assay. A 60-bp-long subfragment of this region was found to interact with nuclear proteins, including high-mobility-group (HMG) proteins, and with the maize HMGa protein synthesized in Escherichia coli from a recombinant plasmid. The potential influence of the binding of the HMG proteins on the conformation of this subfragment was studied with a permutation assay based on a bending vector.
来自玉米（Zea mays L. cv. A619）核rRNA基因单元的3kb外部间隔区包含9个高度同源的200bp重复元件，该间隔区被发现包含一个具有DNA弯曲特性的区域。使用环形置换分析，曲率中心定位在重复序列5和6内。发现该区域一个60bp长的亚片段与包括高迁移率族（HMG）蛋白在内的核蛋白以及从重组质粒在大肠杆菌中合成的玉米HMGa蛋白相互作用。基于弯曲向量的置换分析研究了HMG蛋白结合对该亚片段构象的潜在影响。
