School of Environment, Harbin Institute of Technology, 73 Huanghe road, Harbin, Heilongjiang, 150090, China.
School of Environment, Harbin Institute of Technology, 73 Huanghe road, Harbin, Heilongjiang, 150090, China.
J Hazard Mater. 2018 Oct 15;360:471-480. doi: 10.1016/j.jhazmat.2018.08.032. Epub 2018 Aug 16.
Organics metabolic function of bacterial communities was evaluated in 60 days composting of swine manure and pumice by using MiSeq sequencing, PICRUSt and Biolog tools. The diversity of bacterial communities significantly decreased during the first 10 days, and gradually increased in the cooling and curing phase. The PICRUSt and Biolog analysis indicated that carbohydrate, lipid and amino acids metabolisms were relatively higher in the thermophilic phases. Xenobiotics biodegradation and metabolism, lipid metabolism, terpenoids and polyketides and biosynthesis of other secondary metabolites were mainly detected in the curing phases. Canonical correspondence analysis (CCA) indicated that the succession of bacterial community and organics utilization characteristics were highly affected by the temperature, moisture and oxidation reduction potential (ORP) in the swine composting system.
采用 MiSeq 测序、 PICRUSt 和 Biolog 工具，评估了猪粪和浮石在 60 天堆肥过程中的细菌群落有机代谢功能。在最初的 10 天内，细菌群落的多样性显著下降，然后在冷却和固化阶段逐渐增加。PICRUSt 和 Biolog 分析表明，在高温阶段碳水化合物、脂质和氨基酸代谢相对较高。在固化阶段，主要检测到了外源生物降解和代谢、脂质代谢、萜类和聚酮以及其他次生代谢物的生物合成。典型对应分析（CCA）表明，细菌群落的演替和有机物利用特性受猪堆肥系统温度、水分和氧化还原电位（ORP）的强烈影响。
