Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) , Nanjing Tech University (NanjingTech) , 30 South Puzhu Road , Nanjing , 211816 , P. R. China.
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering , Zhejiang University of Technology , Chaowang Road 18 , Hangzhou 310014 , P. R. China.
ACS Sens. 2018 Sep 28;3(9):1622-1626. doi: 10.1021/acssensors.8b00456. Epub 2018 Aug 30.
Reactive sulfur species play a very important role in modulating neural signal transmission. Hydrogen polysulfides (HS , n > 1) are recently suggested to be the actual signaling molecules. There are still few spatiotemporal controllable-based probes to detect HS . In this work, for the first time, we proposed the photocleavage product of the common photoremovable protecting group (2-nitrophenyl moiety) capable of trapping HS . Taking advantage of this, we constructed the probe H1 containing a photocontrollable group, a mitochondrial directing unit and a signal reporter fluorescein dye. H1 exhibited excellent fluorescence enhancement (50-fold) in response to HS under the aqueous buffer only after UV irradiation. H1 also showed high selectivity and sensitivity for HS over other reactive sulfur species, reactive oxygen species, and other analytes, especially biothoils including hydrogen sulfide, cysteine, homocysteine, and glutathione. We showed the utility of H1 to image HS in living cells with high spatiotemporal resolution.
活性硫物种在调节神经信号转导方面起着非常重要的作用。氢多硫化物（HS，n>1）最近被认为是实际的信号分子。目前仍然很少有基于时空可控的探针来检测 HS。在这项工作中，我们首次提出了一种能够捕获 HS 的常见光脱保护基（2-硝基苯基部分）的光裂解产物。利用这一点，我们构建了含有光控基团、线粒体靶向单元和信号报告荧光染料的探针 H1。H1 在仅经 UV 照射后的水缓冲液中对 HS 表现出优异的荧光增强（50 倍）。H1 对 HS 表现出高选择性和灵敏度，优于其他活性硫物种、活性氧物种和其他分析物，特别是包括硫化氢、半胱氨酸、同型半胱氨酸和谷胱甘肽在内的生物体液。我们展示了 H1 在活细胞中以高时空分辨率成像 HS 的实用性。
