Salih Serwa Ibrahim, Al-Falahi Nadia H, Saliem Ali H, Abedsalih Ahmed N
Department of Surgery and Obstetrics, University of Baghdad, Baghdad, Iraq.
Department of Physiology, Biochemistry and Pharmacology, University of Baghdad, Baghdad, Iraq.
Vet World. 2018 Jul;11(7):944-952. doi: 10.14202/vetworld.2018.944-952. Epub 2018 Jul 16.
The current study was conducted to evaluate the effect of platelet-rich fibrin matrix (PRFM) treated with silver nanoparticles (AgNPs) on enhancing the healing of the experimentally induced bone gap in a rabbit model.
Twenty healthy male local rabbits aged between 6 and 8 months, their weights between 1.5 and 2 kg were used in this study and divided randomly into four equal groups, under general anesthesia (1 cm), bone gap was induced in the tibia bone to create a critical bone defect and leave it without any treatment in the first group (control group). While in the second group the bone gap was filled with PRFM; in the third group, the gap was filled with 0.3 ml AgNPs; and in the fourth group, the gap was filled with PRFM treated with AgNPs.
There was no infection at the operation site in all experimental animals, and the radiograph images showed periosteal and endosteal reaction; the gaps were bridged faster in the fourth group as compared with the other groups. The histological examination showed lamellar bone with haversian canal completely filled the fracture gap and contact with old bone in the fourth group as compared to other groups.
Using a combination of PRFM and single nucleotide polymorphisms together gave better acceleration in the bone healing process than using each one of them separately.
本研究旨在评估经银纳米颗粒（AgNPs）处理的富血小板纤维蛋白基质（PRFM）对兔模型中实验性诱导骨间隙愈合的促进作用。
本研究使用20只6至8个月大、体重在1.5至2千克之间的健康雄性本地兔，将其随机分为四组，在全身麻醉下（1厘米），在胫骨中诱导骨间隙以制造临界骨缺损，第一组（对照组）不进行任何处理。第二组用PRFM填充骨间隙；第三组用0.3毫升AgNPs填充间隙；第四组用经AgNPs处理的PRFM填充间隙。
所有实验动物的手术部位均未发生感染，X线片显示有骨膜和骨内膜反应；与其他组相比，第四组的间隙更快被桥接。组织学检查显示，与其他组相比，第四组中具有哈弗斯管的板层骨完全填充了骨折间隙并与旧骨接触。
与单独使用PRFM和单核苷酸多态性中的任何一种相比，将它们联合使用能更好地加速骨愈合过程。
