Bukrinskaia A G, Gracheva N M, Starov A I, Moĭsiadi S A, Blokhina T A
Vopr Virusol. 1986 Mar-Apr;31(2):197-200.
A method of rapid diagnosis of rotavirus infections is described. It is based on the isolation of viral RNA from the feces of patients with viral gastroenteritis and determination of RNA mobility by 6% polyacrylamide gel electrophoresis. It was demonstrated that rotavirus infection could be diagnosed after long-term storage of fecal specimens as well. The method is of interest for epidemiological and genetic studies of human rotavirus diseases.
本文描述了一种轮状病毒感染的快速诊断方法。该方法基于从病毒性肠胃炎患者的粪便中分离病毒RNA,并通过6%聚丙烯酰胺凝胶电泳测定RNA的迁移率。结果表明,粪便标本长期保存后也可诊断轮状病毒感染。该方法对人类轮状病毒疾病的流行病学和遗传学研究具有重要意义。