Rohollahzadeh Hadi, Nili Hassan, Asasi Keramat, Mokhayeri Saeed, Najjari Amir Hossein Asl
1Resident of Poultry Science, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran.
2Isfahan Univeristy, Isfahan, Iran.
Comp Clin Path. 2018;27(5):1241-1255. doi: 10.1007/s00580-018-2728-z. Epub 2018 May 9.
Newcastle disease causes a lymphoproliferative response in the tracheal and intestinal mucosa of the infected birds. In this study, the Hitchner B1 and I-2 vaccine and challenging of ND field strains were used to evaluate the populations of T lymphocyte subsets infiltrated intestinal and tracheal, also to shed some light on cell-mediated immune response using enzyme-linked immunosorbent assay (ELISA) detecting chicken's serum interferon-γ. Three hundred-day-old broilers were randomly divided into four groups. Groups 1 and 2 received I-2 and B1 vaccines, respectively, while groups 3 and 4 were challenged-unvaccinated and unchallenged-unvaccinated groups. Blood samples were taken from five random chicks and were then tested with ELISA test. Three chicks of each group were euthanized after vaccine administration and also challenging with acute virus. Interferon-γ changes were significant in time ( < 0.001). Totally, there was no significant difference between I-2 and B1 groups. The number of CD3+, CD4+, and CD8+ cells of I-2 and B1 vaccinated group's intestine and the trachea samples was significantly increased compared with the negative control group ( < 0.001). The results indicated the significant increase in CD4+ and CD8+ in intestinal and tracheal tissues, while the level of interferon-γ of the vaccinated group was more than the unvaccinated one. Finding no significant differences between the vaccinated groups indicated the potential of both vaccines in producing CD4+ and CD8+ in the tracheal and intestinal tissues and the equality of interferon-γ production in the sera.
新城疫会在受感染禽类的气管和肠道黏膜中引发淋巴细胞增殖反应。在本研究中，使用希钦纳B1和I-2疫苗以及新城疫野毒株攻毒来评估浸润肠道和气管的T淋巴细胞亚群数量，同时通过酶联免疫吸附测定（ELISA）检测鸡血清中的干扰素-γ，以深入了解细胞介导的免疫反应。将300只日龄肉鸡随机分为四组。第1组和第2组分别接种I-2和B1疫苗，而第3组和第4组分别为攻毒未免疫组和未攻毒未免疫组。从五只随机选取的雏鸡采集血样，然后用ELISA检测。每组三只雏鸡在接种疫苗以及用急性病毒攻毒后实施安乐死。干扰素-γ的变化具有显著的时间差异（<0.001）。总体而言，I-2组和B1组之间没有显著差异。与阴性对照组相比，接种I-2和B1疫苗组的肠道和气管样本中CD3 +、CD4 +和CD8 +细胞数量显著增加（<0.001）。结果表明肠道和气管组织中CD4 +和CD8 +显著增加，而接种疫苗组的干扰素-γ水平高于未接种组。接种疫苗组之间未发现显著差异，表明两种疫苗在气管和肠道组织中产生CD4 +和CD8 +的潜力以及血清中干扰素-γ产生的等同性。
