Hoornstra Douwe, Vesterlin Jenni, Pärnänen Pirjo, Al-Samadi Ahmed, Zlotogorski-Hurvitz Ayelet, Vered Marilena, Salo Tuula
Department of Oral and Maxillofacial Diseases, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Department of Oral Pathology and Oral Medicine, School of Dental Medicine, Tel Aviv University, Tel Aviv, Israel.
In Vivo. 2018 Sep-Oct;32(5):1089-1095. doi: 10.21873/invivo.11350.
Oral tongue squamous cell carcinoma (OTSCC) cells are highly proliferative and invasive. Lingonberry contains several polyphenolic compounds similar to curcumin. We hypothesize that fermented lingonberry juice (FLJ) has an anti-invasive and anti-proliferative effect on OTSCC cells similarly to curcumin, which is known to be anti-carcinogenic.
FLJ, curcumin dissolved in ethanol, or curcumin loaded in Candida extracellular vesicles (EVs) were added to more (HSC-3) and less aggressive (SCC-25) OTSCC cells. Cell proliferation was measured with a 5-bromo-2'-deoxyuridine kit and invasion in the three-dimensional Myogel spheroid assay. Statistical analyses were completed with one-way ANOVA and Bonferroni post-hoc testing.
Both FLJ and curcumin significantly reduced the proliferation and invasion of HSC-3 and SCC-25 cells. The effects of curcumin were not improved when cells were treated with curcumin loaded within EVs.
Our results suggest that FLJ, like curcumin, has an anti-carcinogenic effect on aggressive OTSCC cells in vitro.
口腔舌鳞状细胞癌（OTSCC）细胞具有高度增殖性和侵袭性。越橘含有几种类似于姜黄素的多酚类化合物。我们假设发酵越橘汁（FLJ）对OTSCC细胞具有与姜黄素类似的抗侵袭和抗增殖作用，而姜黄素已知具有抗癌作用。
将FLJ、溶解于乙醇的姜黄素或负载于念珠菌细胞外囊泡（EVs）中的姜黄素添加到侵袭性较强的（HSC-3）和侵袭性较弱的（SCC-25）OTSCC细胞中。使用5-溴-2'-脱氧尿苷试剂盒测量细胞增殖，并通过三维Myogel球体试验检测细胞侵袭。采用单因素方差分析和Bonferroni事后检验进行统计分析。
FLJ和姜黄素均显著降低了HSC-3和SCC-25细胞的增殖和侵袭。当用负载于EVs中的姜黄素处理细胞时，姜黄素的效果并未得到改善。
我们的结果表明，FLJ与姜黄素一样，在体外对侵袭性OTSCC细胞具有抗癌作用。
