Montgomery R D, Villegas P, Dawe D L, Brown J
Avian Dis. 1986 Apr-Jun;30(2):298-308.
Reovirus 81-176 was inoculated subcutaneously into day-old specific-pathogen-free leghorns and evaluated for its effects on the immune system over a 3-week period. Structural criteria included organ weights of the bursa of Fabricius (BF) and spleen (SP), scoring of histological lesions in the BF, SP, and thymus, and hematological analyses of the circulating leukocytes. Alterations in the functional capacity of the immune system were measured using the graft-versus-host reaction, the response of peripheral blood lymphocytes (PBLs) to mitogens, the ability of circulating monocytes to phagocytize latex beads, and the serological responses to Newcastle disease virus, sheep red blood cells, and Brucella abortus antigens. For comparison, infectious bursal disease virus (IBDV) was similarly evaluated by most of the same tests. Structurally, reovirus 81-176 altered BF and SP organ weights, the total numbers of white blood cells in circulation, and the degree of follicular atrophy in the BF. Functionally, reovirus inoculation reduced both the response of PBLs to the phytohemagglutinin-P stimulation and monocyte uptake of latex beads. According to the protocols used here, no significant alteration in B-cell function could be detected in reovirus-infected chicks. With the exception of leukocyte hematology, IBDV-infected chicks had significantly altered responses in all tests used. By way of comparison, the effects of IBDV were more persistent and pronounced than were those seen with reovirus. The graft-versus-host reaction indicated an elevated and/or uninhibited response of T-cells in the blood of IBDV-infected chicks.
将呼肠孤病毒81 - 176皮下接种到1日龄无特定病原体的来航鸡中，并在3周时间内评估其对免疫系统的影响。结构标准包括法氏囊（BF）和脾脏（SP）的器官重量、BF、SP和胸腺组织学损伤的评分以及循环白细胞的血液学分析。使用移植物抗宿主反应、外周血淋巴细胞（PBL）对丝裂原的反应、循环单核细胞吞噬乳胶珠的能力以及对新城疫病毒、绵羊红细胞和布鲁氏菌流产抗原的血清学反应来测量免疫系统功能能力的变化。为了进行比较，传染性法氏囊病病毒（IBDV）也通过大多数相同的测试进行了类似评估。在结构上，呼肠孤病毒81 - 176改变了BF和SP的器官重量、循环中白细胞的总数以及BF中滤泡萎缩的程度。在功能上，接种呼肠孤病毒降低了PBL对植物血凝素-P刺激的反应以及单核细胞对乳胶珠的摄取。根据这里使用的方案，在感染呼肠孤病毒的雏鸡中未检测到B细胞功能有明显改变。除白细胞血液学外，在所有使用的测试中，感染IBDV的雏鸡的反应都有显著改变。相比之下，IBDV的影响比呼肠孤病毒更持久、更明显。移植物抗宿主反应表明感染IBDV的雏鸡血液中T细胞的反应增强和/或未受抑制。
