College of Animal Science and Technology, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, People's Republic of China.
Biol Trace Elem Res. 2019 May;189(1):186-193. doi: 10.1007/s12011-018-1467-y. Epub 2018 Aug 27.
Oxidative stress is involved in the regulation of mammalian reproduction. The present study was conducted to detect the sodium arsenite-induced oxidative stress and alterations in the structure and steroidogenesis in rat ovary. Twenty female adult rats were injected i.p. with sodium arsenite (8 mg/kg BW, T) or 0.9% saline (C) for 16 days. The oxidative stress indexes and morphology of the liver, kidney, and ovary were detected using commercial kits and HE staining, respectively. The serum progesterone and estradiol were detected by RIA, and the ovarian steroidogenic gene expressions were detected by real-time PCR. Results showed that the ovarian activities of SOD and GSH-PX decreased (P < 0.05), while the ROS activity and MDA level increased (P < 0.05) in the T group. HE staining results showed that treatment with sodium arsenite damaged the ovarian morphology, resulting in reduced large and medium follicles and increased atretic follicles. Nonetheless, neither the liver nor kidney showed evident changes in the oxidative stress indexes or morphology after sodium arsenite treatment. The serum progesterone and estradiol levels decreased (P < 0.05) with the reduced expressions in the ovarian steroidogenic genes (StAR, P450scc, and 3β-HSD) (P < 0.05). In conclusion, sodium arsenite injection can induce ovarian oxidative stress in rats which set up an appropriate model for future studies of ovarian diseases as well as the toxic mechanism of arsenic in the reproduction.
氧化应激参与了哺乳动物生殖的调控。本研究旨在检测亚砷酸钠诱导的氧化应激以及对大鼠卵巢结构和类固醇生成的改变。将 20 只成年雌性大鼠经腹腔注射亚砷酸钠（8mg/kg BW，T 组）或 0.9%生理盐水（C 组）16 天。使用商业试剂盒和 HE 染色分别检测肝脏、肾脏和卵巢的氧化应激指标和形态。采用 RIA 检测血清孕激素和雌二醇水平，实时 PCR 检测卵巢类固醇生成基因的表达。结果显示，T 组大鼠卵巢中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性降低（P<0.05），而活性氧和 MDA 水平升高（P<0.05）。HE 染色结果显示，亚砷酸钠处理导致卵巢形态受损，大、中卵泡减少，闭锁卵泡增多。然而，肝脏和肾脏在氧化应激指标或形态上均未显示出明显的变化。血清孕激素和雌二醇水平降低（P<0.05），同时卵巢类固醇生成基因（StAR、P450scc 和 3β-HSD）的表达减少（P<0.05）。综上，亚砷酸钠注射可诱导大鼠卵巢氧化应激，为今后研究卵巢疾病以及砷在生殖系统中的毒性机制提供了合适的模型。
