Klibet Fahima, Boumendjel Amel, Khiari Mohamed, El Feki Abdelfattah, Abdennour Cherif, Messarah Mahfoud
a Laboratory of Biochemistry and Environmental Toxicology , and.
b Applied Biochemistry and Microbiology Laboratory, Faculty of Sciences , University of Badji Mokhtar , Annaba , Algeria .
Pharm Biol. 2016;54(2):354-63. doi: 10.3109/13880209.2015.1043562. Epub 2015 May 6.
Pistacia lentiscus L. (Anacardiaceae) is an evergreen shrub widely distributed throughout the Mediterranean region. Pistacia lentiscus oil (PLo) was particularly known in North African traditional medicine. Thus, people of these regions have used it externally to treat sore throats, burns and wounds, as well as they employed it internally for respiratory allergies. PLo is rich in essential fatty acids, vitamin E and polyphenols. As a very active site of metabolism, liver is reported to be susceptible to arsenic (As) intoxication.
The present study evaluates the protective effect of PLo against sodium arsenite-induced hepatic dysfunction and oxidative stress in experimental Wistar rats.
Twenty-eight rats were equally divided into four groups; the first served as a control, the remaining groups were respectively treated with PLo (3.3 mL/kg body weight), sodium arsenite (5.55 mg/kg body weight) and a combination of sodium arsenite and PLo. After 21 consecutive days, cellular functions were evaluated by hematological, biochemical and oxidative stress markers.
A significant decrease in the levels of red blood cells, haemoglobin (p ≤ 0.001), hematocrit (p ≤ 0.001), reduced glutathione and metallothionein (p ≤ 0.05) associated with a significant increase of malondialdehyde (p ≤ 0.001) were noticed in the arsenic-exposed group when compared to the control. The As-treated group also exhibited an increase in hepatic antioxidant enzymes namely superoxide dismutase, glutathione peroxidase (p ≤ 0.01) and catalase (p ≤ 0.05). However, the co-administration of PLo has relatively reduced arsenic effect.
The results showed that arsenic intoxication disturbed the liver pro-oxidant/antioxidant status. PLo co-administration mitigates arsenic-induced oxidative damage in rat.
乳香黄连木（漆树科）是一种常绿灌木，广泛分布于地中海地区。乳香黄连木油（PLo）在北非传统医学中尤为知名。因此，这些地区的人们外用它来治疗喉咙痛、烧伤和伤口，也内服它来治疗呼吸道过敏。PLo富含必需脂肪酸、维生素E和多酚。肝脏作为一个非常活跃的代谢部位，据报道易受砷（As）中毒的影响。
本研究评估PLo对实验性Wistar大鼠亚砷酸钠诱导的肝功能障碍和氧化应激的保护作用。
28只大鼠平均分为四组；第一组作为对照组，其余各组分别用PLo（3.3毫升/千克体重）、亚砷酸钠（5.55毫克/千克体重）以及亚砷酸钠和PLo的组合进行处理。连续21天后，通过血液学、生化和氧化应激标志物评估细胞功能。
与对照组相比，砷暴露组的红细胞、血红蛋白（p≤0.001）、血细胞比容（p≤0.001）、还原型谷胱甘肽和金属硫蛋白水平显著降低（p≤0.05），丙二醛水平显著升高（p≤0.001）。砷处理组的肝脏抗氧化酶即超氧化物歧化酶、谷胱甘肽过氧化物酶（p≤0.01）和过氧化氢酶（p≤0.05）也有所增加。然而，PLo的联合使用相对减轻了砷的作用。
结果表明，砷中毒扰乱了肝脏的促氧化剂/抗氧化剂状态。PLo的联合使用减轻了大鼠体内砷诱导的氧化损伤。
