PASTEUR, Département de Chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France.
Bioessays. 2018 Oct;40(10):e1800118. doi: 10.1002/bies.201800118. Epub 2018 Aug 28.
Fluorescence imaging has become an indispensable tool in cell and molecular biology. GFP-like fluorescent proteins have revolutionized fluorescence microscopy, giving experimenters exquisite control over the localization and specificity of tagged constructs. However, these systems present certain drawbacks and as such, alternative systems based on a fluorogenic interaction between a chromophore and a protein have been developed. While these systems are initially designed as fluorescent labels, they also present new opportunities for the development of novel labeling and detection strategies. This review focuses on new labeling protocols, actuation methods, and biosensors based on fluorogenic protein systems.
荧光成像是细胞和分子生物学中不可或缺的工具。GFP 样荧光蛋白彻底改变了荧光显微镜技术，使实验人员能够对标记构建体的定位和特异性进行精细控制。然而，这些系统存在某些缺点，因此，基于发色团和蛋白质之间的荧光相互作用的替代系统已经得到开发。虽然这些系统最初被设计为荧光标记物，但它们也为开发新的标记和检测策略提供了新的机会。本综述重点介绍了基于荧光蛋白系统的新型标记方案、致动方法和生物传感器。
