Neurath A R, Kent S B, Strick N, Parker K
Cell. 1986 Aug 1;46(3):429-36. doi: 10.1016/0092-8674(86)90663-x.
Hepatitis B virus (HBV) has not yet been propagated in vitro, and knowledge concerning its reaction with receptors on target cells remained scant. We have located within the HBV envelope proteins a sequence mediating the attachment of HBV to human hepatoma HepG2 cells. A synthetic peptide analog (PLGFFPDHQLDPAFGANSNNPDWDFNP) is recognized by both cell receptors and anti-HBV antibodies and elicits antibodies reacting with native HBV. The synthetic peptide is a promising immunogen expected to elicit protective antibodies based on the concept of the attachment blockade pathway of virus neutralization. The approach described here, based on anti-peptide antisera and the binding of peptide analogs to cell receptors is generally applicable for the delineation of cell receptor binding sites on viruses with known gene sequences.
乙型肝炎病毒（HBV）尚未在体外进行繁殖，关于其与靶细胞上受体反应的知识仍然匮乏。我们在HBV包膜蛋白中定位到了一段介导HBV与人肝癌HepG2细胞附着的序列。一种合成肽类似物（PLGFFPDHQLDPAFGANSNNPDWDFNP）可被细胞受体和抗HBV抗体识别，并能引发与天然HBV反应的抗体。基于病毒中和的附着阻断途径概念，该合成肽有望成为一种能引发保护性抗体的免疫原。本文所述方法基于抗肽抗血清以及肽类似物与细胞受体的结合，普遍适用于确定具有已知基因序列病毒的细胞受体结合位点。
