Sigal N H
J Exp Med. 1977 Jul 1;146(1):282-6. doi: 10.1084/jem.146.1.282.
Monoclonal anti-dinitrophenyl antibodies generated in the splenic focus system from B cells of adult BALB/c mice were studied for the presence or absence of murine anti-T15 (M anti-T15) reactivity and for their ability to bind phosphorylcholine (PC). Two foci of the 680 clones analyzed bound PC, and one of these antibodies reacted with M anti-T15 and anti-Fab on a 1:1 weight basis. The discovery of a clonotype reactive with M anti-15 but not with rabbit anti-T15 (R anti-T15) serum, the converse of the R anti-T15+, M anti-T15- clonotype identified in the PC-specific repertoire, points to the novel idiotypic relationships which may be found among homogeneous antibodies binding diverse antigens. The R anti-T15-, M anti-T15+ clonotype may represent a distinct set of hypervariable region sequences inserted into the T15 framework or may be a somatic variant of the T15 germ-line sequence. In addition, the maximum frequency with which this clonotype occurs within the B-cell pool is estimated.
对成年BALB/c小鼠脾脏中由B细胞在脾脏聚焦系统产生的单克隆抗二硝基苯基抗体进行了研究，以确定其是否具有鼠抗T15（M抗T15）反应性及其结合磷酸胆碱（PC）的能力。在分析的680个克隆中，有两个聚焦点与PC结合，其中一种抗体与M抗T15和抗Fab以1:1的重量比发生反应。发现了一种与M抗15反应但不与兔抗T15（R抗T15）血清反应的克隆型，这与在PC特异性库中鉴定出的R抗T15 +、M抗T15 -克隆型相反，这表明在结合不同抗原的同源抗体之间可能存在新的独特型关系。R抗T15 -、M抗T15 +克隆型可能代表插入T15框架的一组独特的高变区序列，或者可能是T15种系序列的体细胞变体。此外，还估计了该克隆型在B细胞库中出现的最大频率。
