Claflin J L, Davie J M
J Exp Med. 1974 Sep 1;140(3):673-86. doi: 10.1084/jem.140.3.673.
A new idiotypic determinant(s) on mouse anti-PC antibodies is described. Antibodies to the determinant(s) were raised in rabbits by immunization with HOPC 8, a PC-binding myeloma protein, and were isolated from HOPC 8 immunoadsorbent by elution with PC. These antibodies react with binding site determinants on anti-PC antibodies raised in all 15 inbred mouse strains tested regardless of histocompatibility or allotype, but fail to react with antibodies of other specificities or with anti-PC antibodies raised in other rodent species. These results correlate closely with other studies which show similar binding specificity of anti-PC antibodies raised in 17 different strains of mice. The site-associated idiotypic determinant(s) is clearly distinct from that detected by mouse anti-HOPC 8 antisera. This latter determinant(s) is present on anti-PC antibodies of only a few strains of mice and may not be in the binding site.
本文描述了小鼠抗磷脂酰胆碱(PC)抗体上一种新的独特型决定簇。通过用PC结合性骨髓瘤蛋白HOPC 8免疫家兔,产生了针对该决定簇的抗体,并通过用PC洗脱从HOPC 8免疫吸附剂中分离出来。这些抗体与在所有15种测试的近交小鼠品系中产生的抗PC抗体上的结合位点决定簇发生反应,而不论其组织相容性或同种异型如何,但不与其他特异性抗体或在其他啮齿动物物种中产生的抗PC抗体发生反应。这些结果与其他研究密切相关,其他研究表明在17种不同小鼠品系中产生的抗PC抗体具有相似的结合特异性。与位点相关的独特型决定簇明显不同于小鼠抗HOPC 8抗血清所检测到的决定簇。后一种决定簇仅存在于少数品系小鼠的抗PC抗体上,且可能不在结合位点。