Nowak Agnieszka, Przywara-Chowaniec Brygida, Damasiewicz-Bodzek Aleksandra, Janoszka Beata, Szumska Magdalena, Waligóra Sławomir, Tyrpień-Golder Krystyna
Department of Chemistry, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland.
2 Department of Cardiology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland.
Arch Med Sci. 2024 Jan 31;20(3):743-750. doi: 10.5114/aoms/176941. eCollection 2024.
Systemic lupus erythematosus (SLE) is a chronic, autoimmune disease, often characterised by severe course and unclear etiopathogenesis. The reaction of protein glycoxidation, also known as glycation, may be linked to etiopathogenesis of SLE. Advanced glycation end-products (AGEs) exhibit cytotoxic properties, affect cellular signalling, impair functions of extracellular proteins, and may act as neoepitopes. Glucosone (GS), glyoxal (GO), and methylglyoxal (MGO) are examples of α-dicarbonyl compounds (α-DCs) partaking in glycoxidation. The study aimed to evaluate concentrations of these three compounds in blood serum of SLE patients, and to compare the results with healthy individuals.
31 women suffering from SLE and 26 healthy individuals were included in the study. High-performance liquid chromatography with fluorescence detection was applied to evaluate concentrations of α-DCs in their serum samples. Correlations between the results and parameters such as disease duration time, age, glomerular filtration rate (GFR), Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K), and creatinine were analysed.
The SLE patients exhibited lower concentrations of glucosone, glyoxal, and methylglyoxal than the control group. Analysis of correlations showed a difference between the examined groups.
In women suffering from SLE the course of α-DCs metabolism is altered. SLE patients are characterised by low serum levels of α-DCs. We hypothesise that either hindered proteasomal degradation or fast consumption of α-DCs in oxidative conditions may cause the observed low concentration of these compounds.
系统性红斑狼疮（SLE）是一种慢性自身免疫性疾病，通常病程严重且病因发病机制不明。蛋白质糖氧化反应，也称为糖化反应，可能与SLE的病因发病机制有关。晚期糖基化终产物（AGEs）具有细胞毒性，影响细胞信号传导，损害细胞外蛋白质的功能，并可能作为新抗原表位。葡萄糖酮（GS）、乙二醛（GO）和甲基乙二醛（MGO）是参与糖氧化反应的α-二羰基化合物（α-DCs）的例子。本研究旨在评估SLE患者血清中这三种化合物的浓度，并将结果与健康个体进行比较。
本研究纳入了31名SLE女性患者和26名健康个体。采用高效液相色谱-荧光检测法评估其血清样本中α-DCs的浓度。分析结果与疾病持续时间、年龄、肾小球滤过率（GFR）、2000年系统性红斑狼疮疾病活动指数（SLEDAI-2K）和肌酐等参数之间的相关性。
SLE患者的葡萄糖酮、乙二醛和甲基乙二醛浓度低于对照组。相关性分析显示两组之间存在差异。
SLE女性患者的α-DCs代谢过程发生改变。SLE患者的特征是血清α-DCs水平较低。我们推测，蛋白酶体降解受阻或氧化条件下α-DCs的快速消耗可能导致观察到的这些化合物浓度较低。
