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具有高灵敏度和低灵敏度位点的单一近红外荧光探针，用于以不同荧光信号模式检测不同浓度范围的生物硫醇。

Single near-infrared fluorescent probe with high- and low-sensitivity sites for sensing different concentration ranges of biological thiols with distinct modes of fluorescence signals.

作者信息

Chen Hua, Tang Yonghe, Ren Mingguang, Lin Weiying

机构信息

State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry and Chemical Engineering , Hunan University , Changsha , Hunan 410082 , China . Email:

Institute of Fluorescent Probes for Biological Imaging , School of Chemistry and Chemical Engineering , School of Biological Science and Technology , University of Jinan , Jinan , Shandong 250022 , P. R. China.

出版信息

Chem Sci. 2016 Mar 1;7(3):1896-1903. doi: 10.1039/c5sc03591k. Epub 2015 Nov 24.

DOI:10.1039/c5sc03591k
PMID:30155014
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6090520/
Abstract

We describe a unique approach for the development of an interesting type of the fluorescent probes, which can show different modes of fluorescence signals to distinct concentration ranges of a target of interest. The key points for the design of the new type of the fluorescent probes include the judicious selection of the dye platforms and the corresponding high- and low-sensitivity sites. It is known that the normal concentrations of biological thiols have significant biological functions. However, up- or down-regulated concentrations of thiols may induce several diseases. Therefore, it is highly important to monitor the changes of thiol concentrations in living systems. Based on the proposed strategy, we engineer the novel NIR fluorescent probe, , which remarkably can display a turn-on signal to the low concentration range of thiols and a ratiometric response to the high concentration range of thiols for the first time. We anticipate that the intriguing strategy formulated herein will be widely useful for the development of concentration range-dependent fluorescent probes.

摘要

我们描述了一种开发有趣类型荧光探针的独特方法，该探针可以针对目标物的不同浓度范围显示不同模式的荧光信号。新型荧光探针设计的关键点包括对染料平台以及相应的高灵敏度和低灵敏度位点的明智选择。已知生物硫醇的正常浓度具有重要的生物学功能。然而，硫醇浓度的上调或下调可能引发多种疾病。因此，监测生物系统中硫醇浓度的变化非常重要。基于所提出的策略，我们设计了新型近红外荧光探针，该探针首次能够对低浓度硫醇显示开启信号，并对高浓度硫醇显示比率响应。我们预计本文制定的有趣策略将广泛应用于浓度范围依赖性荧光探针的开发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/bc6f7fbb1bdb/c5sc03591k-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/9f62848074d3/c5sc03591k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/c3780e01682a/c5sc03591k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/3d40bb8ae23a/c5sc03591k-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/e86f9dd6ee05/c5sc03591k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/81c7445555a8/c5sc03591k-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/6b6858b47383/c5sc03591k-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/ba6810ccc44e/c5sc03591k-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/563d2b1c9823/c5sc03591k-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/f32f8cd72f42/c5sc03591k-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/138db1e8f64b/c5sc03591k-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/147527ae1225/c5sc03591k-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/bc6f7fbb1bdb/c5sc03591k-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/9f62848074d3/c5sc03591k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/c3780e01682a/c5sc03591k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/3d40bb8ae23a/c5sc03591k-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/e86f9dd6ee05/c5sc03591k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/81c7445555a8/c5sc03591k-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/6b6858b47383/c5sc03591k-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/ba6810ccc44e/c5sc03591k-s2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/563d2b1c9823/c5sc03591k-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/f32f8cd72f42/c5sc03591k-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/138db1e8f64b/c5sc03591k-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/147527ae1225/c5sc03591k-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9650/6090520/bc6f7fbb1bdb/c5sc03591k-f10.jpg

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