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通过生物正交受激拉曼散射显微镜成像药物摄取。

Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy.

作者信息

Tipping William J, Lee Martin, Serrels Alan, Brunton Valerie G, Hulme Alison N

机构信息

EaStCHEM School of Chemistry , The University of Edinburgh , Joseph Black Building, David Brewster Road , Edinburgh , EH9 3FJ , UK . Email:

Edinburgh Cancer Research Centre , Institute of Genetics and Molecular Medicine , The University of Edinburgh , Crewe Road South , Edinburgh , EH4 2XR , UK . Email:

出版信息

Chem Sci. 2017 Aug 1;8(8):5606-5615. doi: 10.1039/c7sc01837a. Epub 2017 May 24.

DOI:10.1039/c7sc01837a
PMID:30155229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6103005/
Abstract

Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation of a series of Raman-active labels has allowed the identification of highly active labels which have minimal perturbation on the biological efficacy of the parent drug. Drug uptake has been correlated with markers of cellular composition and cell cycle status, and mapped across intracellular structures using dual-colour and multi-modal imaging. The minimal phototoxicity and low photobleaching associated with SRS microscopy has enabled real-time imaging in live cells. These studies demonstrate the potential for SRS microscopy in the drug development process.

摘要

受激拉曼散射（SRS）显微镜与生物正交拉曼标记策略相结合，有望彻底改变细胞内药物摄取的直接可视化。对一系列拉曼活性标记物的合理评估，已确定了高活性标记物，这些标记物对母体药物的生物学功效影响最小。药物摄取已与细胞组成和细胞周期状态的标志物相关联，并通过双色和多模态成像在细胞内结构上进行了映射。SRS显微镜相关的最小光毒性和低光漂白特性，使得在活细胞中进行实时成像成为可能。这些研究证明了SRS显微镜在药物开发过程中的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46d0/6103005/9919256ddbaf/c7sc01837a-f8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46d0/6103005/9919256ddbaf/c7sc01837a-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46d0/6103005/09c23755ed37/c7sc01837a-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46d0/6103005/14a2f89dfdff/c7sc01837a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46d0/6103005/2e5113faeb47/c7sc01837a-f6.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/46d0/6103005/9919256ddbaf/c7sc01837a-f8.jpg

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