Ralph P, Kishimoto T
J Clin Invest. 1981 Oct;68(4):1093-6. doi: 10.1172/jci110332.
Immunoglobulin production by lymphoblast cell lines was studies using protein A-red blood cell plaque formation to detect individual secreting cells. Immunoglobulin (Ig) secretion by 6 of 12 human B-cell lines tested could be stimulated up to twentyfold by phorbol myristic acetate (PMA) at subtoxic concentrations of 10-1000 ng/ml depending on the line. Stimulation was found with both IgM and IgG cell lines. No switch of Ig class synthesis was found in the cell lines as a result of PMA incubation. Increase in Ig secretion was closely associated with cessation of growth resembling induction of terminal differentiation in the cells. PMA induction of Ig secretion in B lymphocytes from normal peripheral blood requires the cooperation of T cells. PMA stimulation of certain cell lines reported here suggests that the lines are late in the differentiation pathway to plasmacyte and can be easily triggered to secrete Ig by membrane-altering agents.
利用蛋白A-红细胞空斑形成法检测单个分泌细胞,对淋巴母细胞系的免疫球蛋白产生情况进行了研究。在所检测的12个人B细胞系中,有6个系的免疫球蛋白(Ig)分泌可被佛波酯(PMA)在10 - 1000 ng/ml的亚毒性浓度下刺激至高达20倍,具体倍数取决于细胞系。IgM和IgG细胞系均出现了刺激现象。经PMA孵育后,未发现细胞系中Ig类合成发生转换。Ig分泌增加与细胞生长停止密切相关,类似于细胞终末分化的诱导。PMA诱导正常外周血B淋巴细胞分泌Ig需要T细胞的协同作用。此处报道的对某些细胞系的PMA刺激表明,这些细胞系处于向浆细胞分化途径的后期,并且可以很容易地被改变细胞膜的试剂触发分泌Ig。