Asakawa Yuki, Takesue Nobuchika, Asano Shizuka, Shimotsu Satoshi, Iijima Kazumaru, Suzuki Koji, Motoyama Yasuo, Aizawa Masayuki
Research Laboratories for Alcohol Beverages, Asahi Breweries, Ltd, 1-21, Midori 1-chome, Moriya-shi, Ibaraki, 302-0106, Japan.
Quality Control Center, Asahi Breweries, Ltd, 1-21, Midori 1-chome, Moriya-shi, Ibaraki, 302-0106, Japan.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3899-3906. doi: 10.1099/ijsem.0.002220. Epub 2017 Sep 12.
A Gram-stain-positive, catalase-negative and short-rod-shaped organism, designated VTT E-94560, was isolated from beer in Finland and deposited in the VTT culture collection as a strain of Lactobacillus rossiae. However, the results of 16S rRNA gene sequence analysis showed that VTT E-94560 was only related to Lactobacillus rossiae JCM 16176 with 97.0 % sequence similarity, lower than the 98.7 % regarded as the boundary for the species differentiation. Additional phylogenetic studies on the pheS gene, rpoA gene and 16S-23S rRNA internally transcribed spacer region further reinforced the taxonomically independent status of VTT E-94560 and its related Lactobacillus species including L. rossiae and Lactobacillus siliginis. Strain VTT E-94560 also exhibited several differences in its carbohydrate fermentation profiles from those related Lactobacillus species. In addition, DNA-DNA relatedness between VTT E-94560 and these two type strains was 4 % (L. rossiae JCM 16176) and 12 % (L. siliginins JCM 16155), respectively, which were lower than the 70 % cut-off for general species delineation, indicating that these three strains are not taxonomically identical at the species level. These studies revealed that VTT E-94560 represents a novel species, for which the name Lactobacillus curtus sp. nov. is proposed. The type strain is VTT E-94560 (=JCM 31185).
从芬兰啤酒中分离出一株革兰氏染色阳性、过氧化氢酶阴性的短杆状菌，命名为VTT E-94560，并作为罗西亚乳杆菌菌株保藏于VTT培养物保藏中心。然而，16S rRNA基因序列分析结果表明，VTT E-94560与罗西亚乳杆菌JCM 16176的序列相似性仅为97.0%，低于被视为物种分化界限的98.7%。对pheS基因、rpoA基因和16S-23S rRNA内转录间隔区的进一步系统发育研究进一步强化了VTT E-94560及其相关乳杆菌属物种（包括罗西亚乳杆菌和西里吉尼乳杆菌）在分类学上的独立地位。菌株VTT E-94560在碳水化合物发酵谱方面也与这些相关乳杆菌属物种存在若干差异。此外，VTT E-94560与这两个模式菌株的DNA-DNA相关性分别为4%（罗西亚乳杆菌JCM 16176）和12%（西里吉尼乳杆菌JCM 16155），均低于一般物种界定的70%阈值，表明这三个菌株在物种水平上分类不同。这些研究表明，VTT E-94560代表一个新物种，为此提出新名称短乳杆菌（Lactobacillus curtus sp. nov.）。模式菌株为VTT E-94560（=JCM 31185）。
