Doijad Swapnil P, Poharkar Krupali V, Kale Satyajit B, Kerkar Savita, Kalorey Dewanand R, Kurkure Nitin V, Rawool Deepak B, Malik Satya Veer Singh, Ahmad Rafed Yassin, Hudel Martina, Chaudhari Sandeep P, Abt Birte, Overmann Jörg, Weigel Markus, Hain Torsten, Barbuddhe Sukhadeo B, Chakraborty Trinad
1Institute for Medical Microbiology, Justus-Liebig University, and German Center for Infection Research (DZIF), Partner Site Gießen-Marburg-Langen, Campus Gießen, Germany.
2Department of Pathology, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University, Nagpur 440006, India.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3285-3291. doi: 10.1099/ijsem.0.002980. Epub 2018 Aug 29.
Two Listeria-like isolates obtained from mangrove swamps in Goa, India were characterized using polyphasic combinations of phenotypic, chemotaxonomic and whole-genome sequence (WGS)-based approaches. The isolates presented as short, non-spore-forming, Gram-positive rods, that were non-motile, oxidase-negative, catalase-positive and exhibited α-haemolysis on 5 % sheep- and horse-blood agar plates. The 16S rRNA gene sequences exhibited 93.7-99.7 % nucleotide identity to other Listeria species and had less than 92 % nucleotide identity to species of closely related genera, indicating that the isolates are de facto members of the genus Listeria. Their overall fatty acid composition resembled that of other Listeria species, with quantitative differences in iso C15 : 0, anteiso C15 : 0, iso C16 : 0, C16 : 0, iso C17 : 0 and anteiso C17 : 0 fatty acid profiles. Phylogeny based on 406 core coding DNA sequences grouped these two isolates in a monophyletic clade within the genus Listeria. WGS-based average nucleotide identity and in silico DNA-DNA hybridization values were lower than the recommended cut-off values of 95 and 70 %, respectively, to the other Listeria species, indicating that they are founding members of a novel Listeria species. We suggest the name Listeriagoaensis sp. nov. be created and the type strain is ILCC801 (=KCTC 33909;=DSM 29886;=MCC 3285).
从印度果阿邦红树林沼泽中分离出的两株类李斯特菌属菌株，采用表型、化学分类学和基于全基因组序列（WGS）的多相组合方法进行了特征分析。这些分离株呈现为短的、无芽孢形成的革兰氏阳性杆菌，不运动，氧化酶阴性，过氧化氢酶阳性，并且在5%绵羊血和马血琼脂平板上表现出α溶血。16S rRNA基因序列与其他李斯特菌属物种的核苷酸同一性为93.7 - 99.7%，与密切相关属的物种的核苷酸同一性低于92%，表明这些分离株实际上是李斯特菌属的成员。它们的总体脂肪酸组成与其他李斯特菌属物种相似，但在异C15:0、anteiso C15:0、异C16:0、C16:0、异C17:0和anteiso C17:0脂肪酸谱上存在定量差异。基于406个核心编码DNA序列的系统发育分析将这两株分离株归为李斯特菌属内的一个单系分支。基于WGS的平均核苷酸同一性和电子DNA - DNA杂交值分别低于与其他李斯特菌属物种推荐的95%和70%的临界值，表明它们是一个新的李斯特菌属物种的奠基成员。我们建议创建新物种名称Listeriagoaensis sp. nov.，模式菌株为ILCC801（=KCTC 33909；=DSM 29886；=MCC 3285）。
