Department of Food Science, Cornell University, Ithaca, NY 14853, USA.
Life Technologies, 2130 Woodward Street, Austin, TX 78744, USA.
Int J Syst Evol Microbiol. 2014 Jun;64(Pt 6):1882-1889. doi: 10.1099/ijs.0.052720-0. Epub 2014 Mar 5.
Sampling of agricultural and natural environments in two US states (Colorado and Florida) yielded 18 Listeria-like isolates that could not be assigned to previously described species using traditional methods. Using whole-genome sequencing and traditional phenotypic methods, we identified five novel species, each with a genome-wide average BLAST nucleotide identity (ANIb) of less than 85% to currently described species. Phylogenetic analysis based on 16S rRNA gene sequences and amino acid sequences of 31 conserved loci showed the existence of four well-supported clades within the genus Listeria; (i) a clade representing Listeria monocytogenes, L. marthii, L. innocua, L. welshimeri, L. seeligeri and L. ivanovii, which we refer to as Listeria sensu stricto, (ii) a clade consisting of Listeria fleischmannii and two newly described species, Listeria aquatica sp. nov. (type strain FSL S10-1188(T) = DSM 26686(T) = LMG 28120(T) = BEI NR-42633(T)) and Listeria floridensis sp. nov. (type strain FSL S10-1187(T) = DSM 26687(T) = LMG 28121(T) = BEI NR-42632(T)), (iii) a clade consisting of Listeria rocourtiae, L. weihenstephanensis and three novel species, Listeria cornellensis sp. nov. (type strain TTU A1-0210(T) = FSL F6-0969(T) = DSM 26689(T) = LMG 28123(T) = BEI NR-42630(T)), Listeria grandensis sp. nov. (type strain TTU A1-0212(T) = FSL F6-0971(T) = DSM 26688(T) = LMG 28122(T) = BEI NR-42631(T)) and Listeria riparia sp. nov. (type strain FSL S10-1204(T) = DSM 26685(T) = LMG 28119(T) = BEI NR- 42634(T)) and (iv) a clade containing Listeria grayi. Genomic and phenotypic data suggest that the novel species are non-pathogenic.
在美国的两个州（科罗拉多州和佛罗里达州）的农业和自然环境中采样，得到了 18 株无法通过传统方法分配到已描述物种的李斯特菌样分离株。使用全基因组测序和传统表型方法，我们鉴定了 5 个新种，每个种的全基因组平均 BLAST 核苷酸同一性（ANIb）均低于目前描述的物种的 85%。基于 16S rRNA 基因序列和 31 个保守基因座的氨基酸序列的系统发育分析表明，李斯特菌属内存在四个支持良好的分支；（i）代表李斯特菌单核细胞增生李斯特菌、L. marthii、L. innocua、L. welshimeri、L. seeligeri 和 L. ivanovii 的分支，我们称之为李斯特菌属严格意义上的李斯特菌，（ii）由李斯特菌 fleischmannii 和两个新描述的种组成的分支，李斯特菌水生亚种。新种（模式株 FSL S10-1188（T）=DSM 26686（T）=LMG 28120（T）=BEI NR-42633（T））和李斯特菌佛罗里达亚种。新种（模式株 FSL S10-1187（T）=DSM 26687（T）=LMG 28121（T）=BEI NR-42632（T）），（iii）由李斯特菌 rocourtiae、李斯特菌 weihenstephanensis 和三个新种组成的分支，李斯特菌科内利斯亚种。新种（模式株 TTU A1-0210（T）=FSL F6-0969（T）=DSM 26689（T）=LMG 28123（T）=BEI NR-42630（T））、李斯特菌 grandensis 新种（模式株 TTU A1-0212（T）=FSL F6-0971（T）=DSM 26688（T）=LMG 28122（T）=BEI NR-42631（T））和李斯特菌 riparia 新种（模式株 FSL S10-1204（T）=DSM 26685（T）=LMG 28119（T）=BEI NR-42634（T）），以及（iv）包含李斯特菌 grayi 的分支。基因组和表型数据表明，这些新种是非致病性的。
