Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular de Insetos, Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro, Instituto de Bioquímica Médica Leopoldo de Meis, Laboratório de Bioquímica de Insetos Hematófagos, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2018 Aug 23;113(10):e180290. doi: 10.1590/0074-02760180290.
Zika has emerged as a new public health threat after the explosive epidemic in Brazil in 2015. It is an arbovirus transmitted mainly by Aedes aegypti mosquitoes. The knowledge of physiological, behavioural and biological features in virus-infected vectors may help the understanding of arbovirus transmission dynamics and elucidate their influence in vector capacity.
We aimed to investigate the effects of Zika virus (ZIKV) infection in the behaviour of Ae. aegypti females by analysing the locomotor activity, egg production and viability.
Ae. aegypti females were orally infected with ZIKV through an artificial feeder to access egg production, egg viability and locomotor activity. For egg production and viability assays, females were kept in cages containing an artificial site for oviposition and eggs were counted. Locomotor activity assays were performed in activity monitors and an average of 5th, 6th and 7th days after infective feeding was calculated.
No significant difference in the number of eggs laid per females neither in their viability were found between ZIKV infected and non-infected females, regardless the tested pair of mosquito population and virus strain and the gonotrophic cycles. Locomotor activity assays were performed regardless of the locomotor activity in ZIKV infected females was observed, in both LD and DD conditions.
The lower locomotor activity may reduce the mobility of the mosquitoes and may explain case clustering within households reported during Zika outbreaks such as in Rio de Janeiro 2015. Nevertheless, the mosquitoes infected with ZIKV are still able to disseminate and to transmit the disease, especially in places where there are many oviposition sites.
2015 年巴西爆发的疫情使寨卡病毒成为新的公共卫生威胁。它是一种主要通过埃及伊蚊传播的虫媒病毒。了解病毒感染媒介的生理、行为和生物学特征有助于理解虫媒病毒的传播动态，并阐明其对媒介容量的影响。
我们旨在通过分析运动活动、产卵和存活率，研究寨卡病毒（ZIKV）感染对埃及伊蚊雌性行为的影响。
通过人工饲养器，将雌性埃及伊蚊经口感染寨卡病毒，以获取产卵、卵活力和运动活动数据。对于产卵和活力检测，将雌性饲养在包含人工产卵点的笼子中，计数产卵数量。运动活动检测在活动监测器中进行，计算感染后第 5、6、7 天的平均活动。
无论测试的蚊群和病毒株以及生殖周期如何，感染寨卡病毒的雌性与未感染的雌性相比，产卵数量和卵活力均无显著差异。无论在 LD 还是 DD 条件下，是否观察到感染寨卡病毒的雌性的运动活动，均进行了运动活动检测。
较低的运动活动可能会降低蚊子的活动性，这可以解释 2015 年在里约热内卢爆发的寨卡疫情中报告的病例在家庭内聚集的情况。然而，感染寨卡病毒的蚊子仍能够传播和传播疾病，特别是在有许多产卵点的地方。
