鼠伤寒沙门氏菌体内DNA聚合酶IIIγ亚基与β亚基的相互作用
Interaction of DNA polymerase III gamma and beta subunits in vivo in Salmonella typhimurium.
作者信息
Engstrom J, Wong A, Maurer R
出版信息
Genetics. 1986 Jul;113(3):499-515. doi: 10.1093/genetics/113.3.499.
Abstract
We show that temperature-sensitive mutations in dnaZ, the gene for the gamma subunit of DNA polymerase III holoenzyme, can be suppressed by mutations in the dnaN gene, which encodes the beta subunit. These results support a direct physical interaction of these two subunits during polymerase assembly or function. The suppressor phenotype is also sensitive to modulation by the dnaA genotype. Since dnaA is organized in an operon with dnaN, and dnaA is a regulatory gene of this operon, we propose that the dnaA effect on suppression can best be explained by modulation of suppressor dnaN levels.
摘要
我们发现，DNA聚合酶III全酶γ亚基的编码基因dnaZ中的温度敏感突变可被编码β亚基的dnaN基因中的突变所抑制。这些结果支持了这两个亚基在聚合酶组装或功能过程中存在直接的物理相互作用。抑制子表型也对dnaA基因型的调节敏感。由于dnaA与dnaN位于同一个操纵子中，且dnaA是该操纵子的一个调控基因，我们提出，dnaA对抑制作用的影响最好通过调节抑制子dnaN的水平来解释。
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本文引用的文献
1
2
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J Biol Chem. 1982 May 25;257(10):5692-9.
3
4
Purification and characterization of the beta subunit of the DNA polymerase III holoenzyme of Escherichia coli.大肠杆菌DNA聚合酶III全酶β亚基的纯化与特性分析
J Biol Chem. 1980 Nov 25;255(22):10984-90.
5
Coordinate expression of Escherichia coli dnaA and dnaN genes.大肠杆菌dnaA和dnaN基因的协同表达。
Mol Gen Genet. 1980;179(3):521-6. doi: 10.1007/BF00271741.
6
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7
Genetic approaches to the analysis of microbial development.微生物发育分析的遗传学方法。
Annu Rev Genet. 1982;16:61-83. doi: 10.1146/annurev.ge.16.120182.000425.
8
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.pUC质粒，一种源自M13mp7的用于插入诱变和使用合成通用引物进行测序的系统。
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9
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