Ismail Howaida I H, Ashour Dalia S, Saad Abeer E
J Egypt Soc Parasitol. 2017 Apr;47(1):197-206.
Giardiasis is one of the most frequent entero-parasites worldwide; its prevalence is more common in developing countries. Giardia lamblia is considered one of the opportunistic parasites and aits clinical manifestations represent the expression of host resistance and parasite viri lence. As of chronic giardiasis cases were expected to worsen in immunosuppressed patients with development of various complications. This work assessed the histolopathological and the possible immunological effects of infection with G. lamblia in an immunosuppressed experimental animal model in comparison to immunocompetent one. Mice were divided into 4 groups;. group I: simmunocompetent (IC) mice infected with G. lamblia cysts, group II: immunosup pressed (S) mice infected with cysts, group III: uninfected immunocompetent mice and group. IV: uninfected immunosuppressed mice. From each group, small intestine was. removed for histopathological and molecular studies. Also, cyst counting in the stool of infected mice was estimated. We found that the number of G. lamblia cysts in the stool of IS mice was significantly higher than that from IC ones. Shortening of the villi was more pronounced in the IS than in IC group. Furthermore, intraepithelial lymphocytic count, goblet cell count and mast cell count were significantly decreased in the IS infected group as compared to IC ones. Expression of interleukin.6 mRNA showed high expression in IC infected mice while it was weak in IS mice. In conclusicn, the present study revealed the importance of the innate immunity presented by goblet cells, mast cells and other adaptive immunity responses; in the clearance of Giardia.
贾第虫病是全球最常见的肠道寄生虫病之一；其在发展中国家更为普遍。蓝氏贾第鞭毛虫被认为是机会性寄生虫之一，其临床表现代表了宿主抵抗力和寄生虫毒力的表现。由于慢性贾第虫病病例预计在免疫抑制患者中会随着各种并发症的发展而恶化。这项工作评估了与免疫健全的实验动物模型相比，蓝氏贾第鞭毛虫感染在免疫抑制实验动物模型中的组织病理学和可能的免疫效应。将小鼠分为4组；第一组：感染蓝氏贾第鞭毛虫包囊的免疫健全（IC）小鼠，第二组：感染包囊的免疫抑制（S）小鼠，第三组：未感染的免疫健全小鼠，第四组：未感染的免疫抑制小鼠。从每组中取出小肠进行组织病理学和分子研究。此外，还估计了感染小鼠粪便中的包囊计数。我们发现，免疫抑制小鼠粪便中的蓝氏贾第鞭毛虫包囊数量明显高于免疫健全小鼠。免疫抑制组绒毛缩短比免疫健全组更明显。此外，与免疫健全组相比，免疫抑制感染组的上皮内淋巴细胞计数、杯状细胞计数和肥大细胞计数明显减少。白细胞介素-6 mRNA的表达在免疫健全感染小鼠中高表达，而在免疫抑制小鼠中弱表达。总之，本研究揭示了杯状细胞、肥大细胞呈现的固有免疫和其他适应性免疫反应在清除贾第虫中的重要性。
