Yassien N A, el-Kowrany S E, Atta F A, Ghoraba H M
Department of Parasitology, Tanta University, Egypt.
J Egypt Soc Parasitol. 1996 Dec;26(3):661-9.
This study was carried out on sixty mice which were allocated in two group. Group I: included thirty mice which were splenectomized and orally inoculated with Giardia lamblia cysts. Group II: thirty intact infected mice which served as a control group. The mice in group I captured G. lamblia infection and demonstrated trophozoites in their intestinal sections when PAS (periodic acid Schiff) and IIF (indirect immunofluorescence) stains. On the other hand, intestinal sections stained with hematoxyline and eosin showed a spectrum of pathological changes with shortened fused villi, heavy lymphocytic infiltration in the lamina propria and atrophy in musculosa and ganglion cells which showed decrease in number per base of villus. The levels of antibody response measured with IIF-test showed significant decrease in group I compared with the control group. This study demonstrated the role of humoral immunity in infection with G. lamblia.
本研究对60只小鼠进行，将其分为两组。第一组：包括30只接受脾切除并经口接种蓝氏贾第鞭毛虫包囊的小鼠。第二组：30只完整的感染小鼠作为对照组。第一组小鼠感染蓝氏贾第鞭毛虫，在其肠道切片经PAS（过碘酸希夫）和IIF（间接免疫荧光）染色时可见滋养体。另一方面，苏木精和伊红染色的肠道切片显示出一系列病理变化，包括绒毛缩短融合、固有层大量淋巴细胞浸润以及肌层和神经节细胞萎缩，每根绒毛基部的神经节细胞数量减少。用IIF试验测量的抗体反应水平显示，与对照组相比，第一组显著降低。本研究证明了体液免疫在蓝氏贾第鞭毛虫感染中的作用。
