Attia Marwa M, Saad M F, Abdel-Salam Ayah B
J Egypt Soc Parasitol. 2017 Apr;47(1):227-234.
The present study determined the milk value rather than serum in diagnosis of toxoplasmosis in goats using ELISA and Modified Agglutination Test (MAT). ELISA proved to be more specific and sensitive (92% & 85%), than MAT (90% & 80%) in diagnosis of Anti-T. gondii Antibodies (ATAb) in group of microscopically proved T gondii aborted goats respectively. There was a direct relationship between.the mean ELISA O.D. value and the level of Ab-titer in MAT positive sera. The overall prevalence of infection in 600 serum samples from grazing goats was 22% and 20% after examination using ELISA and IAT respectively. It was lower after examination of their milk (20%) and (18%) by both techniques respectively. ATAb were significant high in serum (P < 0.05) during January, February and March, decreased significantly in April (P < 0.05) till August, then significant increased (P < 0.05) from September to December using ELISA. In time where ATAb were present by different levels among the whole year in serum, it didn't diagnose in milk from June to September. The infection was high in Egyptian Baladi breed in serum and milk (30% and 27%), while the Barki breed was the lowest one (13.33% and 12%) respectively. High rate of infection (30% in serum and 27% in milk) was recorded in goats > 2 years old, while no ATAb could be detected in goats less than one year old. The highest prevalence of infection (39.28% in serum and 37.14% in milk) was in Qalyoubia, the lowest one (11.25% & 9.58%) was in Giza governorate. The prevalence of infection was high after examination of serum and milk of goats with previous history of abortion in comparison with the other goats.
本研究使用酶联免疫吸附测定法（ELISA）和改良凝集试验（MAT），确定了山羊弓形虫病诊断中乳汁而非血清的价值。在经显微镜检查证实为弓形虫流产的山羊群体中，ELISA在诊断抗弓形虫抗体（ATAb）方面比MAT更具特异性和敏感性（分别为92%和85%，而MAT为90%和80%）。在MAT阳性血清中，ELISA平均光密度值与抗体滴度水平之间存在直接关系。对600份放牧山羊血清样本进行检测后，ELISA和间接血凝试验（IAT）检测到的总体感染率分别为22%和20%。通过两种技术分别检测其乳汁后，感染率较低（分别为20%和18%）。使用ELISA检测时，1月、2月和3月血清中的ATAb显著升高（P<0.05），4月显著下降（P<0.05）直至8月，然后从9月至12月显著升高（P<0.05）。在全年血清中存在不同水平ATAb的时期，6月至9月乳汁中未检测到感染。埃及巴拉迪品种山羊血清和乳汁中的感染率较高（分别为30%和27%），而巴尔基品种最低（分别为13.33%和12%）。2岁以上山羊的感染率较高（血清中为30%，乳汁中为27%），而1岁以下山羊未检测到ATAb。感染率最高的是盖勒尤比省（血清中为39.28%；乳汁中为37.14%），最低的是吉萨省（11.25%和9.58%）。与其他山羊相比，有流产史的山羊血清和乳汁检测后的感染率较高。
