State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, China; Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, China Agricultural University, Beijing 100193, China; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Infect Genet Evol. 2018 Nov;65:392-398. doi: 10.1016/j.meegid.2018.08.026. Epub 2018 Aug 26.
Extraintestinal pathogenic Escherichia coli (ExPEC) are important human pathogens responsible for urinary tract infection and meningitis. Therefore, infection of chickens by highly pathogenic E. coli with multi-drug resistance has become a major concern to food safety. In this study, we isolated a strain of E. coli (HB2016) from the oviduct of a diseased chicken with colibacillosis. Inoculation of chickens with 2 × 10 CFU of the isolate E. coli HB2016 by intraperitoneal injection successfully reproduced colibacillosis in chickens. We also found that E. coli HB2016 harbored four more virulence genes (tsh, trat, cvaC and cvaA/B) than E. coli reference strain CVCC1428. Importantly, E. coli HB2016 was resistant to cefuroxime, tobramycin, medemycin, cefazolin, cefoperazone, streptomycin and ampicillin, and carried multiple antibiotic resistance genes such as strA, strB, bla, bla, bla, fosA, mph(A), floR, sul2, tet(A) and tet(B). These findings suggest that the causative E. coli act as a potential zoonotic agent affecting human health.
肠外致病性大肠杆菌（ExPEC）是引起尿路感染和脑膜炎的重要人类病原体。因此，高致病性、多重耐药大肠杆菌感染鸡只已成为食品安全的主要关注点。在本研究中，我们从患有大肠杆菌病的鸡输卵管中分离出一株大肠杆菌（HB2016）。通过腹腔注射 2×10 CFU 的分离株大肠杆菌 HB2016 可成功在鸡体内复制大肠杆菌病。我们还发现，HB2016 比大肠杆菌参考株 CVCC1428 多携带 4 个毒力基因（tsh、trat、cvaC 和 cvaA/B）。重要的是，HB2016 对头孢呋辛、妥布霉素、美罗培南、头孢唑林、头孢哌酮、链霉素和氨苄西林耐药，并携带多种抗生素耐药基因，如 strA、strB、bla、bla、bla、fosA、mph(A)、floR、sul2、tet(A)和 tet(B)。这些发现表明，这种致病性大肠杆菌可能成为影响人类健康的潜在人畜共患病原体。
