Liu Shiqi, Liu Junfa, Fu Ning, Kornmatitsuk Bunlue, Yan Zhuanqiang, Luo Junrong
Jiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
Jinzhai County Agriculture and Rural Bureau, Jinzhai, Anhui, China.
Front Microbiol. 2023 Jan 11;13:985789. doi: 10.3389/fmicb.2022.985789. eCollection 2022.
is an important duck pathogen responsible for septicemia and infectious serositis, which has caused great economic losses to the duck industry. Phenylalanine-arginine β-naphthylamide (PAβN) is an efflux pump inhibitor, which mainly reduces the efflux effect by competing with antibiotics for efflux pump channels. Here, we found that strain GD2019 showed resistances to gentamicin, amikacin, kanamycin, and neomycin. Notably, PAβN could significantly reduce the Minimal inhibitory concentrations (MICs) of neomycin on the GD2019 strain. Moreover, PAβN combined with neomycin significantly decreased bacterial loads, relieved pathological injury and increase survival rate ( < 0.05) for the ducks lethally challenged by the GD2019 strain. Therefore, our results suggested, and PAβN could reduce neomycin-resistant of Importantly, finding of this study provide a new approach for treating antibiotic-resistant infection.
是一种重要的鸭病原体，可导致败血症和传染性浆膜炎，给养鸭业造成了巨大的经济损失。苯丙氨酸 - 精氨酸β - 萘酰胺（PAβN）是一种外排泵抑制剂，主要通过与抗生素竞争外排泵通道来降低外排效应。在此，我们发现GD2019菌株对庆大霉素、阿米卡星、卡那霉素和新霉素具有抗性。值得注意的是，PAβN可显著降低新霉素对GD2019菌株的最低抑菌浓度（MIC）。此外，PAβN与新霉素联合使用可显著降低细菌载量，减轻病理损伤，并提高受GD2019菌株致死性攻击的鸭的存活率（<0.05）。因此，我们的结果表明，PAβN可降低对新霉素的耐药性。重要的是，本研究的发现为治疗抗生素耐药性感染提供了一种新方法。
