McCullough K C, Crowther J R, Butcher R N, Carpenter W C, Brocchi E, Capucci L, De Simone F
Immunology. 1986 Jul;58(3):421-8.
Monoclonal antibodies (MAb) against sequential or conformational epitopes on foot-and-mouth disease virus (FMDV) passively protected neonatal syngeneic (BALB/c) mice at dilutions at which they could not neutralize virus infectivity in vitro. The B2, D9, 1C6 and 4C9 MAb, against the Group 1 (sequential) and Group 2 (conformational) epitopes, protected the mice at an antibody:virion molar ratio of between 38:1 and 84:1 (12-18 times lower than that required for neutralization of virus infectivity in vitro). The 3C8 (Group 3) and 6C3 (Group 4) MAb were, respectively, between 5 and 12 times, and between 18 and 40 times, less efficient at protection. There was no consistent correlation between the efficiency of neutralization of virus infectivity in vitro and the protection of neonatal mice against the virus pathogen. Thus, immune protection against FMDV must use mechanisms other than the direct neutralization of virus infectivity by antibody. Complement did not increase the virus neutralization titre of the MAb, but pepsin digestion of the MAb abrogated the enhanced in vivo protection over in vitro neutralization, with little effect on their capacity to neutralize virus infectivity. It is therefore likely that opsonization to a minimum affinity, and subsequent rapid phagocytosis, play a major role in the immune defence against FMDV. This is discussed in terms of the natural host for FMDV and the induction of immunological protection.
针对口蹄疫病毒（FMDV）连续或构象表位的单克隆抗体（MAb），在其体外不能中和病毒感染性的稀释度下，可被动保护同基因新生（BALB/c）小鼠。针对第1组（连续）和第2组（构象）表位的B2、D9、1C6和4C9单克隆抗体，以38:1至84:1的抗体:病毒粒子摩尔比保护小鼠（比体外中和病毒感染性所需的摩尔比低12 - 18倍）。3C8（第3组）和6C3（第4组）单克隆抗体在保护效率上分别低5至12倍和18至40倍。体外中和病毒感染性的效率与新生小鼠抵抗病毒病原体的保护之间没有一致的相关性。因此，针对FMDV的免疫保护必须使用除抗体直接中和病毒感染性之外的机制。补体并未增加单克隆抗体的病毒中和效价，但单克隆抗体经胃蛋白酶消化后，体内保护作用相对于体外中和作用的增强被消除，而对其中和病毒感染性的能力影响很小。因此，以最低亲和力进行调理作用，随后快速吞噬作用，可能在针对FMDV的免疫防御中起主要作用。本文根据FMDV的天然宿主和免疫保护的诱导对此进行了讨论。
