Vandeyar M A, Zahler S A
J Bacteriol. 1986 Aug;167(2):530-4. doi: 10.1128/jb.167.2.530-534.1986.
We describe 46 insertions of the Streptococcus faecalis transposon Tn917 into the chromosome of Bacillus subtilis. These insertion mutations were mapped genetically. Some caused auxotrophic requirements, and others were cryptic. These insertions were scattered around the B. subtilis chromosome. The mutant strains were useful in several ways for mapping and cloning B. subtilis genes and were added to the Bacillus Genetic Stock Center collection. Among the auxotrophic markers were a new serine auxotrophy and deletion-insertions that caused auxotrophy in one case for homoserine and threonine, in another case for uracil and either cysteine or methionine, and in a third case for leucine, isoleucine, and valine.
我们描述了粪肠球菌转座子Tn917插入枯草芽孢杆菌染色体的46个插入事件。这些插入突变通过遗传学方法进行了定位。有些导致营养缺陷型需求，而另一些则是隐性的。这些插入分布在枯草芽孢杆菌染色体上。这些突变菌株在多种方面可用于枯草芽孢杆菌基因的定位和克隆，并已被添加到芽孢杆菌遗传菌种保藏中心的菌种库中。在营养缺陷型标记中，有一个新的丝氨酸营养缺陷型以及缺失插入突变，其中一个缺失插入突变导致高丝氨酸和苏氨酸营养缺陷，另一个导致尿嘧啶和半胱氨酸或甲硫氨酸营养缺陷，第三个导致亮氨酸、异亮氨酸和缬氨酸营养缺陷。
