Jenkins F J, Roizman B
J Virol. 1986 Aug;59(2):494-9. doi: 10.1128/JVI.59.2.494-499.1986.
Herpes simplex virus 1 genome consists of two covalently linked components, L and S, that invert relative to each other to yield four equimolar isomeric populations designated P (prototype), Is (inversion of S component), Il (inversion of L component), and Ils (inversion of L and S components) differing in the orientation of the two components. Previous studies have yielded recombinant genomes frozen in the P isomeric arrangement, reinforcing suggestions that the four isomers may not be functionally equivalent. We report the isolation of recombinants produced by insertional mutagenesis with alpha TK mini-Mu that are frozen in Is and Ils arrangements. Thus, all isomeric forms of herpes simplex virus DNA appear to be capable of independent replication and must be considered as functionally equivalent.
单纯疱疹病毒1型基因组由两个共价连接的组分L和S组成，它们彼此相对反转，产生四个等摩尔的异构群体，分别命名为P（原型）、Is（S组分反转）、Il（L组分反转）和Ils（L和S组分反转），这四个群体在两个组分的方向上有所不同。先前的研究产生了以P异构排列冻结的重组基因组，这进一步表明这四种异构体在功能上可能不等同。我们报告了通过αTK微型Mu插入诱变产生的重组体的分离，这些重组体以Is和Ils排列冻结。因此，单纯疱疹病毒DNA的所有异构形式似乎都能够独立复制，必须被视为在功能上等同。
