Hickey D A, Benkel B F, Abukashawa S, Haus S
Department of Biology, University of Ottawa, Ontario, Canada.
Biochem Genet. 1988 Dec;26(11-12):757-68. doi: 10.1007/BF02395521.
A spontaneous null mutation at the alpha-amylase locus in Drosophila melanogaster was recovered from a laboratory population. The mutant strain was found to lack amylase enzyme production and to produce low, but detectable, levels of amylase mRNA. Moreover, the null strain is also lacking the glucose repression of amylase mRNA production which is seen in wild-type strains. The mutant phenotype correlates with a rearrangement in genomic DNA which, in turn, corresponds to a simple inversion in the arrangement observed most frequently in North American populations of D. melanogaster, including the common laboratory strain, Oregon-R. These results have implications for our understanding of both the evolution of the duplicated amylase gene structure and the regulation of amylase gene expression.
从一个实验室种群中发现了黑腹果蝇α-淀粉酶基因座的一个自发无效突变。该突变菌株被发现缺乏淀粉酶的产生，但能产生低水平但可检测到的淀粉酶信使核糖核酸。此外，该无效菌株也缺乏野生型菌株中所见的淀粉酶信使核糖核酸产生的葡萄糖抑制作用。突变表型与基因组DNA的重排相关，而这种重排又对应于在北美黑腹果蝇种群（包括常见的实验室菌株俄勒冈-R）中最常观察到的排列方式的简单倒位。这些结果对我们理解重复淀粉酶基因结构的进化以及淀粉酶基因表达的调控都有启示。
