黑腹果蝇α-淀粉酶基因的分子克隆。I. 使用小鼠探针分离克隆
Molecular cloning of alpha-amylase genes from Drosophila melanogaster. I. Clone isolation by use of a mouse probe.
作者信息
Gemmill R M, Levy J N, Doane W W
出版信息
Genetics. 1985 Jun;110(2):299-312. doi: 10.1093/genetics/110.2.299.
Abstract
A cloned alpha-amylase cDNA sequence from the mouse is homologous to a small set of DNA sequences from Drosophila melanogaster under appropriate conditions of hybridization. A number of recombinant lambda phage that carry homologous Drosophila genomic DNA sequences were isolated using the mouse clone as a hybridization probe. Putative amylase clones hybridized in situ to one or the other of two distinct sites in polytene chromosome 2R and were assigned to one of two classes, A and B. Clone lambda Dm32, representing class A, hybridizes within chromosome section 53CD. Clone lambda Dm65 of class B hybridizes within section 54A1-B1. Clone lambda Dm65 is homologous to a 1450- to 1500-nucleotide RNA species, which is sufficiently long to code for alpha-amylase. No RNA homologous to lambda Dm32 was detected. We suggest that the class B clone, lambda Dm65, contains the functional Amy structural gene(s) and that class A clones contain an amylase pseudogene.
摘要
在适当的杂交条件下，从小鼠中克隆出的α-淀粉酶cDNA序列与黑腹果蝇的一小部分DNA序列具有同源性。使用小鼠克隆作为杂交探针，分离出了许多携带同源果蝇基因组DNA序列的重组λ噬菌体。推定的淀粉酶克隆原位杂交到多线染色体2R上两个不同位点中的一个或另一个，并被分为A和B两类。代表A类的克隆λDm32在染色体区段53CD内杂交。B类的克隆λDm65在区段54A1 - B1内杂交。克隆λDm65与一种1450至1500个核苷酸的RNA物种同源，其长度足以编码α-淀粉酶。未检测到与λDm32同源的RNA。我们认为B类克隆λDm65包含功能性的Amy结构基因，而A类克隆包含淀粉酶假基因。
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