Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Department of Physics, Harvard University, Cambridge, MA 02138, USA.
Science. 2018 Aug 31;361(6405):880-887. doi: 10.1126/science.aau1044. Epub 2018 Aug 30.
Super-resolution microscopy has overcome a long-held resolution barrier-the diffraction limit-in light microscopy and enabled visualization of previously invisible molecular details in biological systems. Since their conception, super-resolution imaging methods have continually evolved and can now be used to image cellular structures in three dimensions, multiple colors, and living systems with nanometer-scale resolution. These methods have been applied to answer questions involving the organization, interaction, stoichiometry, and dynamics of individual molecular building blocks and their integration into functional machineries in cells and tissues. In this Review, we provide an overview of super-resolution methods, their state-of-the-art capabilities, and their constantly expanding applications to biology, with a focus on the latter. We will also describe the current technical challenges and future advances anticipated in super-resolution imaging.
超分辨率显微镜克服了长期存在的分辨率障碍——光显微镜中的衍射极限——并使以前在生物系统中不可见的分子细节可视化。自发明以来，超分辨率成像方法不断发展，现在可用于以纳米级分辨率对三维、多色和活细胞系统中的细胞结构进行成像。这些方法已被用于回答涉及单个分子构建块的组织、相互作用、化学计量和动态及其在细胞和组织中的功能机制中的整合的问题。在这篇综述中，我们概述了超分辨率方法及其最先进的功能，以及它们在生物学中的不断扩展的应用，重点是后者。我们还将描述超分辨率成像中当前的技术挑战和预期的未来进展。
