Guidobono F, Netti C, Sibilia V, Villa I, Zamboni A, Pecile A
Peptides. 1986 Mar-Apr;7(2):315-22. doi: 10.1016/0196-9781(86)90230-5.
The distribution of binding site for [125I]-eel-calcitonin (ECT) to rat central nervous system, studied by an autoradiographic technique, showed concentrations of binding in the diencephalon, the brain stem and the spinal cord. Large accumulations of grains were seen in the hypothalamus, the amygdala, in the fasciculus medialis prosencephali, in the fasciculus longitudinalis medialis, in the ventrolateral part of the periventricular gray matter, in the lemniscus medialis and in the raphe nuclei. The density of grains in the reticular formation and in the nucleus tractus spinalis nervi trigemini was more moderate. In the spinal cord, grains were scattered throughout the dorsal horns. Binding of the ligand was displaced equally by cold ECT and by salmon CT(sCT), indicating that both peptides bind to the same receptors. Human CT was much weaker than sCT in displacing [125I]-ECT binding. The administration of ECT into the brain ventricles of rats dose-dependently induced a significant and long-lasting enhancement of hot-plate latencies comparable with that obtained with sCT. The antinociceptive activity induced by ECT is compatible with the topographical distribution of binding sites for the peptide and is a further indication that fish CTs are active in the mammalian brain.
采用放射自显影技术研究了[125I]-鳗鱼降钙素（ECT）在大鼠中枢神经系统中的结合位点分布，结果显示在间脑、脑干和脊髓中有结合位点的聚集。在下丘脑、杏仁核、前脑内侧束、内侧纵束、室周灰质腹外侧部分、内侧丘系和中缝核中可见大量银粒聚集。网状结构和三叉神经脊束核中的银粒密度较为适中。在脊髓中，银粒散布于整个背角。冷ECT和鲑鱼降钙素（sCT）对配体结合的置换作用相同，表明这两种肽都与相同的受体结合。人降钙素在置换[125I]-ECT结合方面比sCT弱得多。向大鼠脑室注射ECT可剂量依赖性地显著且持久地延长热板潜伏期，与注射sCT的效果相当。ECT诱导的镇痛活性与该肽结合位点的拓扑分布一致，进一步表明鱼类降钙素在哺乳动物脑中具有活性。
