木霉 GS8-2 诱导的系统抗性对黄瓜花叶病毒的作用促进拟南芥病程相关基因的转录。

Induced systemic resistance against Cucumber mosaic virus by Phoma sp. GS8-2 stimulates transcription of pathogenesis-related genes in Arabidopsis.

机构信息

Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafr El-sheikh, Egypt.

出版信息

Pest Manag Sci. 2019 Mar;75(3):859-866. doi: 10.1002/ps.5193. Epub 2018 Oct 15.

DOI:10.1002/ps.5193

PMID:30168656

Abstract

BACKGROUND

Cucumber mosaic virus (CMV) is a serious threat to vegetable production worldwide. The efficacy of Phoma sp. GS8-2 was evaluated against CMV in Arabidopsis and cucumber plants.

RESULTS

Arabidopsis and cucumber plants treated with barley grain inoculum (BGI) or cell-free filtrate (CF) of GS8-2 demonstrated decreased CMV severity and titre using enzyme-linked immunosorbent assay relative to the control. Cucumber growth and yield parameters were significantly increased due to colonization with GS8-2 under field conditions. Molecular mechanisms underlying mediated resistance induced by GS8-2 against CMV were investigated. Real-time polymerase chain reaction (RT-PCR) results confirmed that both BGI and CF of GS8-2 stimulated the transcription levels of pathogenesis related genes (β1-3 glucanase, chitinase, PR1, PAL1 and LOX1), which could be involved in induced resistance against CMV.

CONCLUSION

摘要

背景

黄瓜花叶病毒（CMV）是全球蔬菜生产的严重威胁。本文评估了木霉 GS8-2 对拟南芥和黄瓜植株中 CMV 的防治效果。

结果

与对照相比，用大麦粒接种物（BGI）或 GS8-2 的无细胞滤液（CF）处理的拟南芥和黄瓜植株，通过酶联免疫吸附试验（ELISA）显示 CMV 严重度和滴度降低。在田间条件下，GS8-2 的定殖显著增加了黄瓜的生长和产量参数。研究了 GS8-2 对 CMV 诱导的介导抗性的分子机制。实时聚合酶链反应（RT-PCR）结果证实，GS8-2 的 BGI 和 CF 均能刺激病程相关基因（β1-3 葡聚糖酶、几丁质酶、PR1、PAL1 和 LOX1）的转录水平，这些基因可能参与了对 CMV 的诱导抗性。