利用制备的 NiO 纳米结构控制黄瓜花叶病毒的新策略。

A new strategy to control Cucumber mosaic virus using fabricated NiO-nanostructures.

机构信息

Pesticides Chemistry and Toxicology Department, Faculty of Agriculture, Kafrelsheikh University, Kafr Elsheikh, 33516, Egypt.

Agricultural Botany Department, Faculty of Agriculture, Kafrelsheikh University, Kafr Elsheikh 33516, Egypt.

出版信息

J Biotechnol. 2019 Dec 20;306:134-141. doi: 10.1016/j.jbiotec.2019.10.003. Epub 2019 Oct 5.

DOI:10.1016/j.jbiotec.2019.10.003

PMID:31593748

Abstract

This study was carried out to fabricate nickel oxide nanostructures (NONS) and to evaluate its ability to control Cucumber mosaic virus (CMV) by direct antiviral activity as well as induction of systemic resistance in treated cucumber plants. The efficacy of nickel oxide nanostructures for control CMV in cucumber plants was biologically evaluated by a reduction in disease severity, reduction in CMV accumulation and expression of regulatory and defense-related genes. Cucumber plants treated with nickel oxide nanostructures showed incredible suppression of CMV infection compared with non-treated plants. The enzyme-linked immunosorbent assay (ELISA) showed a marked reduction in CMV accumulation in cucumber plants treated with nickel oxide nanostructures compared to untreated plants. Based on real-time polymerase chain reaction (RT-PCR) test, cucumber plants treated with nickel oxide nanostructures showed increased expression of regulatory and defense-related genes concerned in salicylic acid (SA) and jasmonic acid (JA)/ethylene (ET) signaling pathways. NONS nanostructures showed direct antiviral activity against CMV resulted in significant reduction in CMV severity and titer relative to untreated plants. Treatment with nickel oxide nanostructures significantly improved cucumber fresh and dry weights as well as number of leaves. The induction of systemic resistance towards CMV by NONS nanostructures considered a novel strategy and first report.

摘要

本研究旨在制备氧化镍纳米结构（NONS），并评估其通过直接抗病毒活性以及诱导处理过的黄瓜植株中的系统抗性来控制黄瓜花叶病毒（CMV）的能力。通过降低疾病严重程度、减少 CMV 积累以及表达调控和防御相关基因，来生物评估氧化镍纳米结构对黄瓜植物中 CMV 的控制效果。与未处理的植物相比，用氧化镍纳米结构处理的黄瓜植物表现出对 CMV 感染的惊人抑制作用。酶联免疫吸附测定（ELISA）显示，与未处理的植物相比，用氧化镍纳米结构处理的黄瓜植物中 CMV 的积累明显减少。基于实时聚合酶链反应（RT-PCR）试验，用氧化镍纳米结构处理的黄瓜植物中与水杨酸（SA）和茉莉酸（JA）/乙烯（ET）信号通路相关的调控和防御相关基因的表达增加。NONS 纳米结构对 CMV 表现出直接的抗病毒活性，导致 CMV 严重度和滴度相对于未处理的植物显著降低。用氧化镍纳米结构处理显著提高了黄瓜的鲜重和干重以及叶片数。通过 NONS 纳米结构诱导对 CMV 的系统抗性被认为是一种新颖的策略和首次报道。