通过酶联免疫吸附测定法对无症状孕妇和新生儿进行单纯疱疹病毒的快速检测。

A rapid detection of herpes simplex virus by ELISA in asymptomatic pregnant women and neonates.

作者信息

Thongkrajai P, Pothinam S, Hahnvajanawong C

出版信息

Southeast Asian J Trop Med Public Health. 1986 Mar;17(1):91-5.

Abstract

Herpes simplex virus (HSV) was detected by the enzyme-linked immunosorbent assay (ELISA). The assay system employed rabbit anti-HSV-2-coated microplates to detect HSV in clinical specimens and the same reagent labelled with peroxidase as a conjugate. The HSV type 2 obtained from vero cell culture and normal cell lysate (NCL) were used as positive and negative reference antigens respectively. HSV was detected in 40 (9.93%) of vaginal swabs obtained from 403 pregnant women just before the deliveries and in 39 (9.68%) fluid samples collected immediately after birth from the mouths of 403 newborns. HSV was detected in five pairs of mother-newborn under investigation. There was no correlation between the incidence of HSV in mothers and newborns (p greater than 0.05).

摘要

采用酶联免疫吸附测定（ELISA）检测单纯疱疹病毒（HSV）。该检测系统使用包被有兔抗HSV - 2的微孔板来检测临床标本中的HSV，并使用标记有过氧化物酶的相同试剂作为结合物。分别将从非洲绿猴肾细胞（Vero）培养物中获得的2型HSV和正常细胞裂解物（NCL）用作阳性和阴性参考抗原。在403名孕妇分娩前采集的阴道拭子中有40份（9.93%）检测到HSV，在403名新生儿出生后立即从口腔采集的39份液体样本中也检测到HSV。在接受调查的五对母婴中检测到了HSV。母亲和新生儿中HSV的发生率之间没有相关性（p大于0.05）。