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[使用酶联免疫吸附测定法和间接荧光抗体技术检测不同年龄组血清中2型单纯疱疹病毒抗体水平]

[Herpes simplex virus type 2 antibody levels in sera from various age groups using ELISA and IFAT].

作者信息

Güngör S, Kocabeyoğlu O, Gün H, Yilmaz E

机构信息

GATA ve As. Tip Fak. Mik. ve Kl. Mik. ABD.

出版信息

Mikrobiyol Bul. 1988 Apr;22(2):113-9.

PMID:2856217
Abstract

We have investigated HSV-2, IgG antibody levels in 180 sera which were obtained from 60 girl students between 18-20 years old, 60 pregnant women between 19-39 years old and 60 young men between 20-22 years old, by using ELISA (Enzyme Linked Immunosorbent Assay) and IFAT (Indirect Fluorescent Antibody Technique). All of the sera obtained from girl students and pregnant women were found negative for HSV-2, IgG antibody by ELISA. However 2 of 60 young men's sera were positive. Totally 2 of 180 sera showed positivity for HSV-2. IgG antibodies (% 1.11). Whilst all of the girl students' sera were negative with IFAT but 4 of 60 pregnant women's sera and 3 of 60 young men's sera were positive with the same test. So, the positivity rate was % 3.89 with IFAT. These findings suggest that HSV-2 infection is not so common in our population.

摘要

我们运用酶联免疫吸附测定法(ELISA)和间接荧光抗体技术(IFAT),对从60名18至20岁女学生、60名19至39岁孕妇以及60名20至22岁年轻男性中采集的180份血清样本进行了单纯疱疹病毒2型(HSV - 2)IgG抗体水平检测。通过ELISA检测发现,所有女学生和孕妇的血清样本中HSV - 2 IgG抗体均呈阴性。然而,60名年轻男性的血清样本中有2份呈阳性。在180份血清样本中,共有2份HSV - 2 IgG抗体呈阳性(占1.11%)。通过IFAT检测,所有女学生的血清样本均为阴性,但60名孕妇的血清样本中有4份呈阳性,60名年轻男性的血清样本中有3份呈阳性。因此,IFAT检测的阳性率为3.89%。这些研究结果表明,HSV - 2感染在我们的人群中并不常见。

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