Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing.
Department of Stomatology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing.
Eur J Orthod. 2019 Aug 8;41(4):333-342. doi: 10.1093/ejo/cjy057.
The role of long non-coding ribonucleic acids (lncRNAs) during orthodontic tooth movement remains unclear. We explored the lncRNA landscape of periodontal ligament stem cells (PDLSCs) subjected to compressive force.
PDLSCs were subjected to static compressive stress (2 g/cm2) for 12 hours. Total RNA was then extracted and sequenced to measure changes in lncRNA and messenger RNA (mRNA) expression levels. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression levels of certain lncRNAs. Differential expression analysis as well as Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were also performed.
In total, 90 lncRNAs and 519 mRNAs were differentially expressed in PDLSCs under compressive stress. Of the lncRNAs, 72 were upregulated and 18 downregulated. The levels of eight lncRNAs of interest (FER1L4, HIF1A-AS2, MIAT, NEAT1, ADAMTS9-AS2, LUCAT1, MIR31HG, and DHFRP1) were measured via qRT-PCR, and the results were found to be consistent with those of RNA sequencing. GO and KEGG pathway analyses showed that a wide range of biological functions were expressed during compressive loading; most differentially expressed genes were involved in extracellular matrix organization, collagen fibril organization, and the cellular response to hypoxia.
The lncRNA expression profile was significantly altered in PDLSCs subjected to compressive stress. These findings expand our understanding of molecular regulation in the mechanoresponse of PDLSCs.
长链非编码核糖核酸（lncRNAs）在正畸牙齿移动过程中的作用尚不清楚。我们探讨了受压力的牙周膜干细胞（PDLSCs）中的 lncRNA 图谱。
将 PDLSCs 置于静态压缩力（2 g/cm2）下 12 小时。然后提取总 RNA 并进行测序，以测量 lncRNA 和信使 RNA（mRNA）表达水平的变化。采用定量实时聚合酶链反应（qRT-PCR）验证某些 lncRNA 的表达水平。还进行了差异表达分析以及基因本体论（GO）和京都基因与基因组百科全书（KEGG）途径分析。
在受压缩力的 PDLSCs 中，总共检测到 90 个 lncRNA 和 519 个 mRNA 的差异表达。在 lncRNAs 中，有 72 个上调，18 个下调。通过 qRT-PCR 测量了 8 个感兴趣的 lncRNA（FER1L4、HIF1A-AS2、MIAT、NEAT1、ADAMTS9-AS2、LUCAT1、MIR31HG 和 DHFRP1）的水平，结果与 RNA 测序一致。GO 和 KEGG 途径分析表明，在压缩加载过程中表达了广泛的生物学功能；大多数差异表达基因参与细胞外基质组织、胶原纤维组织和缺氧细胞反应。
受压力的 PDLSCs 中的 lncRNA 表达谱发生了显著改变。这些发现扩展了我们对 PDLSCs 机械反应中分子调控的理解。
