Yang Zhengbin, Zeng Yongde, Hu Yuedan, Zhou Tingting, Li Jiamin, He Lapin, Zhang Wei, Zeng Xuefeng, Fan Jin
School of Liquor and Food Engineering, Guizhou University, Guiyang, China.
Guizhou Provincial Key Laboratory of Agricultural and Animal Products Storage and Processing, Guiyang, China.
Food Chem X. 2023 Jan 7;17:100570. doi: 10.1016/j.fochx.2023.100570. eCollection 2023 Mar 30.
The antioxidant activity of is associated tightly with its polysaccharide concentration, molar mass and architecture. This study aims to explore the differences in structural and physicochemical traits and oxidation resistances between the polysaccharides from fruit body (ABPs) and mycelial (IAPs) of . The results showed that ABPs and IAPs were constituted by glucose, glucuronic acid, galactose and mannose. However, the molecular weight distribution of IAPs (3.22 × 10 Da (52.73%) and 1.95 × 10 Da (24.71%)) was wider than that of ABPs (5.4 × 10 Da (95.77%)). The shear-thinning performance and viscoelastic behavior of both IAPs and ABPs are representative. IAPs are scattered in sheets, with folds and holes, and have a triple helix structure. ABPs are compact in structure and clear in texture. The main functional groups and thermal stability of both polysaccharides were similar. Concerning the in-vitro oxidation resistance, both of the studied polysaccharides exhibited the potent potential to scavenge hydroxyl radicals (IC = 3.37 ± 0.32 and 6.56 ± 0.54 mg/mL, respectively) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals (IC = 0.89 ± 0.22 and 1.48 ± 0.63 mg/mL, respectively), as well as the moderate reduction power. In addition, IAPs and ABPs were both completely undigested in simulated contexts of saliva, small intestine and stomach, and the two polysaccharide types maintained high DPPH and hydroxyl radical scavenging activities. DDPH scavenging rate during digestion was positively correlated with uronic acid content. To conclude, this study suggests the potential of IAPs as an equivalent alternative to ABPs.
其抗氧化活性与其多糖浓度、摩尔质量和结构密切相关。本研究旨在探讨[具体物种]子实体多糖（ABPs）和菌丝体多糖（IAPs）在结构、物理化学特性及抗氧化性方面的差异。结果表明，ABPs和IAPs均由葡萄糖、葡萄糖醛酸、半乳糖和甘露糖组成。然而，IAPs的分子量分布（3.22×10 Da（52.73%）和1.95×10 Da（24.71%））比ABPs（5.4×10 Da（95.77%））更宽。IAPs和ABPs均具有典型的剪切变稀性能和粘弹性行为。IAPs呈片状分散，有褶皱和孔洞，具有三螺旋结构。ABPs结构紧凑，质地清晰。两种多糖的主要官能团和热稳定性相似。关于体外抗氧化性，两种研究多糖均表现出较强的清除羟基自由基（IC分别为3.37±0.32和6.56±0.54 mg/mL）和1,1-二苯基-2-苦基肼（DPPH）自由基（IC分别为0.89±0.22和1.48±0.63 mg/mL）的潜力，以及中等的还原能力。此外，IAPs和ABPs在唾液、小肠和胃的模拟环境中均完全未被消化，且两种多糖类型均保持较高的DPPH和羟基自由基清除活性。消化过程中的DDPH清除率与糖醛酸含量呈正相关。总之，本研究表明IAPs有潜力作为ABPs的等效替代品。
