Feldman D, Hoar R M, Niemann W H, Valentine T, Cukierski M, Hendrickx A G
Am J Obstet Gynecol. 1986 Aug;155(2):413-24. doi: 10.1016/0002-9378(86)90844-6.
Tubuloreticular inclusions were observed in placental chorionic villi of rhesus monkeys after pregnant female monkeys were injected intramuscularly with recombinant leukocyte A interferon (25 X 10(6) units/kg). They were identified in endothelial cells, fibroblasts, and Hofbauer cells of the chorionic villi, providing evidence that interferon crossed at least part of the maternal-fetal partition. Induction of tubuloreticular inclusions in these cells by exogenous interferon has not been previously reported. Tightly packed regular tubular arrangements appeared in endothelial and Hofbauer cells and loosely organized tubular arrays in fibroblasts. The maximum dimension of the tubuloreticular inclusions measured 3 micron and the diameter of the tubules was 20 nm. The tubuloreticular inclusions were continuous with, and surrounded by, smooth endoplasmic reticulum that was often connected to rough endoplasmic reticulum. The tubuloreticular inclusions were not detected in placental chorionic villi from monkeys not treated with interferon or from interferon-treated monkeys 30 days after cessation of treatment. These results indicate that the formation of tubuloreticular inclusions in rhesus monkey placentas was a transient response associated with elevated serum levels of interferon.
给怀孕的恒河猴肌肉注射重组白细胞A干扰素（25×10⁶单位/千克）后，在其胎盘绒毛膜绒毛中观察到管网状包涵体。在绒毛膜绒毛的内皮细胞、成纤维细胞和霍夫鲍尔细胞中均发现了管网状包涵体，这证明干扰素至少穿过了母胎屏障的一部分。此前尚未有关于外源性干扰素在这些细胞中诱导形成管网状包涵体的报道。在内皮细胞和霍夫鲍尔细胞中出现了紧密排列的规则管状结构，而成纤维细胞中则是排列松散的管状阵列。管网状包涵体的最大尺寸为3微米，小管直径为20纳米。管网状包涵体与光滑内质网相连并被其包围，光滑内质网常与粗面内质网相连。在未用干扰素处理的猴子的胎盘绒毛膜绒毛中，以及在停止治疗30天后接受干扰素治疗的猴子的胎盘绒毛膜绒毛中，均未检测到管网状包涵体。这些结果表明，恒河猴胎盘管网状包涵体的形成是一种与血清干扰素水平升高相关的短暂反应。
