Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Botany, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Microb Pathog. 2018 Dec;125:33-42. doi: 10.1016/j.micpath.2018.08.054. Epub 2018 Aug 29.
Silver nanoparticles, one of the most popular nanomaterials, are used extensively in medicine and industries. The present study biosynthesized spherical Ag/AgCl nanoparticles with a size range of 10-50 nm in less than 5 min. The synthesis was performed in a single step, in a low-cost and eco-friendly manner, from the aqueous extract of Malva Sylvestris leaves. The aqueous extract had a large number of phenolic compounds and carbohydrates as reducing and capping agents. The nanoparticles also showed significant antibacterial and anti-biofilm activities against some multi drug resistant bacteria. They additionally showed antifungal activities on several Candida species. The highest concentration of Ag/AgCl-NPs (62.5 μg/ml) was required in order to inhibit P. aeruginosa B 52, C. glabrata and C. parapsilosis growth. The lowest concentration of Ag/AgCl-NPs (7.8125 μg/ml) inhibited the growth of C. orthopsilosis, P. aeruginosa ATCC 27853 and B. subtilis ATCC 6633. A total of 125 μg/ml of Ag/AgCl-NPs was used to prevent P. aeruginosa B 52 biofilm growth. The concentration of 62.5 μg/ml Ag/AgCl-NPs also eradicated both P. aeruginosa 48 and P. aeruginosa B 52 biofilms. The results showed that Hg and Pb contaminants in water could be colorimetrically detected by these nanoparticles.
银纳米粒子是最受欢迎的纳米材料之一，广泛应用于医学和工业领域。本研究在不到 5 分钟的时间内，以较低的成本和环保的方式，从矢车菊叶的水提物中，一步合成出粒径为 10-50nm 的球形 Ag/AgCl 纳米粒子。水提物中含有大量的酚类化合物和碳水化合物，可作为还原剂和稳定剂。纳米粒子还表现出对一些多药耐药菌的显著抗菌和抗生物膜活性。它们还对几种念珠菌属物种表现出抗真菌活性。为了抑制铜绿假单胞菌 B 52、近平滑念珠菌和近平滑假丝酵母的生长，需要最高浓度的 Ag/AgCl-NPs（62.5μg/ml）。Ag/AgCl-NPs 的最低浓度（7.8125μg/ml）可抑制近平滑念珠菌、铜绿假单胞菌 ATCC 27853 和枯草芽孢杆菌 ATCC 6633 的生长。总共使用 125μg/ml 的 Ag/AgCl-NPs 来防止铜绿假单胞菌 B 52 生物膜的生长。浓度为 62.5μg/ml 的 Ag/AgCl-NPs 也能消除铜绿假单胞菌 48 和铜绿假单胞菌 B 52 生物膜。结果表明，这些纳米粒子可以比色检测水中的汞和铅污染物。
