Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
IET Nanobiotechnol. 2018 Sep;12(6):764-772. doi: 10.1049/iet-nbt.2017.0211.
Silver nanoparticles (AgNPs) have attracted the attention of researchers due to their properties. Biological synthesis of AgNPs is eco-friendly and cost-effective preferred to physical and chemical methods, which utilize environmentally harmful agents and large amounts of energy. Microorganisms have been explored as potential biofactories to synthesize AgNPs. Bacterial NP synthesis is affected by Ag salt concentration, pH, temperature and bacterial species. In this study, spp., isolated from soil, were screened for AgNP synthesis at pH 12 with 5 mM Ag nitrate (AgNO) final concentration at room temperature. The isolate with fastest color change and the best ultraviolet-visible spectrum in width and height were chosen as premier one. AgNO and citrate salts were compared in terms of their influence on NP synthesis. Spherical Ag chloride (AgCl) NPs with a size range of 35-40 nm were synthesized in 1.5 mM Ag citrate solution. Fourier transform infrared analysis demonstrated that protein and carbohydrates were capping agents for NPs. In this study, antimicrobial and antitumor properties of the AgNP were investigated. The resulting AgCl NPs had bacteriostatic activity against four standard spp. And multi-drug resistant strain of . These NPs are also cytotoxic to cancer cell lines MCF-7, U87MG and T293.
银纳米粒子（AgNPs）因其性质而引起了研究人员的关注。与物理和化学方法相比，生物合成 AgNPs 具有环保和经济高效的优势，因为这些方法利用了对环境有害的试剂和大量能源。微生物已被探索作为潜在的生物工厂来合成 AgNPs。细菌 NP 的合成受 Ag 盐浓度、pH 值、温度和细菌种类的影响。在这项研究中，从土壤中分离出的 种被筛选出来，在 pH 值为 12、最终浓度为 5mM 的硝酸银（AgNO）和室温下合成 AgNP。选择颜色变化最快和在宽度和高度上具有最佳紫外可见光谱的分离物作为首选。比较了 AgNO 和柠檬酸盐对 NP 合成的影响。在 1.5mM 的 Ag 柠檬酸盐溶液中合成了粒径范围为 35-40nm 的球形 AgCl NPs。傅里叶变换红外分析表明，蛋白质和碳水化合物是 NPs 的帽状剂。在这项研究中，研究了 AgNP 的抗菌和抗肿瘤特性。所得的 AgCl NPs 对四种标准 种和多药耐药株 具有抑菌活性。这些 NPs 对 MCF-7、U87MG 和 T293 等癌细胞系也具有细胞毒性。
