Toxicology and Diseases Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences (TUMS), Tehran, Iran; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran.
Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran.
Biosens Bioelectron. 2018 Nov 30;120:122-128. doi: 10.1016/j.bios.2018.08.041. Epub 2018 Aug 18.
The present study aimed to develop a highly sensitive label-free electrochemical aptasensor for the detection of Diazinon (DZN), as one of the most widespread organophosphorus compounds. The aptasensor was assembled using screen-printed gold electrode modified by thiolated aptamers which were immobilized on gold nanoparticles (Au NPs). Optimum deposition time, in which the highest electrochemical response occurred, was found in 150 s. Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize electrochemical properties of the novel aptasensor. Electrochemical detection was carried out through differential pulse voltammetry in [Fe(CN)] solution. Fluctuation of the current was examined in the DZN concentration range of 0.1-1000 nM. According to the results, the designed aptasensor provided an extremely lower limit of detection (0.0169 nM) compared with HPLC and other colorimetric techniques for DZN detection. The present highly specific designed aptasensor doesn't interact with other analytes in the real sample. Consequently, the present aptasensor is easy to use and relatively inexpensive with a good sensitivity, stability, and reproducibility for this application. We are now evaluating all approaches to make a portable device for fast and sensitive quantification of DZN and related OPs.
本研究旨在开发一种高灵敏度的无标记电化学适体传感器，用于检测哒嗪磷（DZN），哒嗪磷是最广泛使用的有机磷化合物之一。该适体传感器是通过将巯基化适体固定在金纳米粒子（Au NPs）上修饰的丝网印刷金电极组装而成的。在 150 s 时发现了最佳沉积时间，此时会发生最高的电化学响应。电化学阻抗谱和循环伏安法用于表征新型适体传感器的电化学性质。通过在 [Fe(CN)]溶液中进行差分脉冲伏安法进行电化学检测。在 0.1-1000 nM 的 DZN 浓度范围内检查了电流的波动。根据结果，与 HPLC 和其他用于 DZN 检测的比色技术相比，设计的适体传感器提供了极低的检测限（0.0169 nM）。本研究设计的高特异性适体传感器不会与实际样品中的其他分析物相互作用。因此，本适体传感器具有良好的灵敏度、稳定性和重现性，易于使用且相对便宜，非常适用于该应用。我们目前正在评估所有方法，以便为快速、灵敏地定量 DZN 和相关 OPs 制造便携式设备。
