一种用于检测 ATP 的“开-关”型磷光适体传感器开关。
An ''off-on'' phosphorescent aptasensor switch for the detection of ATP.
机构信息
Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Tianjin Normal University), Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, Tianjin 300387, PR China.
Key Laboratory of Inorganic-Organic Hybrid Functional Materials Chemistry (Tianjin Normal University), Tianjin Key Laboratory of Structure and Performance for Functional Molecule, College of Chemistry, Tianjin Normal University, Tianjin 300387, PR China.
出版信息
Talanta. 2018 Dec 1;190:226-234. doi: 10.1016/j.talanta.2018.07.060. Epub 2018 Jul 20.
An "off-on" phosphorescent aptasensor based on the 3-mercaptopropionic acid (MPA) capped Mn-doped ZnS quantum dots (MPA-Mn:ZnS QDs)/aptamer hybrid system was developed to detect adenosine triphosphate (ATP) in biological fluids. The phosphorescence of MPA-Mn:ZnS QDs was obviously quenched when ATP aptamer was added due to the aggregation induced effect. ATP aptamer, adsorbed on the surface of the phosphorescent MPA-Mn:ZnS QDs, has a high affinity for ATP. And then, with the addition of ATP, phosphorescence was gradually recovered because of the stronger special binding interaction between ATP and ATP aptamer than that between QDs and ATP aptamer. In this case, a high sensitivity and selectivity of phosphorescent aptasensor for the detection of ATP has constructed with a low detection limit of 0.9 nM and a wide linear range from 2 nM to 9 µM. What's more, the phosphorescent aptasensor does not require complex pretreatments and can effectively eliminate the interference from auto fluorescence and scattering light.
一种基于 3-巯基丙酸（MPA）封端的 Mn 掺杂 ZnS 量子点（MPA-Mn:ZnS QDs）/适配体杂交体系的“开-关”磷光适体传感器被开发用于检测生物流体中的三磷酸腺苷（ATP）。当加入 ATP 适体时，由于聚集诱导效应，MPA-Mn:ZnS QDs 的磷光明显猝灭。吸附在磷光 MPA-Mn:ZnS QDs 表面的 ATP 适体与 ATP 具有高亲和力。然后，随着 ATP 的加入，由于 ATP 与 ATP 适体之间的特殊结合相互作用比 QDs 与 ATP 适体之间的特殊结合相互作用更强，磷光逐渐恢复。在这种情况下，构建了一种高灵敏度和选择性的磷光适体传感器用于检测 ATP，其检测限低至 0.9 nM，线性范围从 2 nM 到 9 µM。更重要的是，该磷光适体传感器不需要复杂的预处理，可以有效消除自发荧光和散射光的干扰。
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